FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998791
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC130790.6543455518861151No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC86260.43156087855108416No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAATTT79670.3985909482282039No Hit
TCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC66660.33350160171823867No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA62060.31048768980848923No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61820.3092869639697197No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA57200.28617299157340614No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT55630.2783182433781221No Hit
GTAATAGACGGCCGTGTCTTCAGGACTCAGGCTACTCAACTTCATGTAGA53740.26886252739781197No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT49640.24835012765216574No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA49360.24694928084026793No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG49300.24664909938057555No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC47300.23664305072416278No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG45520.22773766741995535No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC42870.2144796529502084No Hit
CCCCTCTACTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCT42840.2143295622203622No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG42590.21307880613831062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42380.21202817102938726No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA42360.21192811054282315No Hit
GTCTATTACTGTGCGAGAGATGTGATTGGCAGCAGCAGCTGGCTGGTGGG42290.21157789883984868No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG42100.21062732421748948No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41940.20982684032497648No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA41760.2089262959458993No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG41510.20767553986384768No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG41330.20677499548477055No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41020.2052240579430266No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA40090.20057124531779458No Hit
GTAGTAGTCAGCTTCATCCTCAACCTGGGCCCCACTGATAGTCAAGGTGG39400.1971191585313322No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC37520.18771347279430414No Hit
GTAGAGGGGCCTGGCCTGACTTCTGCTGGAACCAGTAAGAATATTTTTTT37170.18596241427943191No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36940.18481171868394444No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG36320.18170984360045647No Hit
CAGTAGTAGTCAGCTTCATCCTCAACCTGGGCCCCACTGATAGTCAAGGT35670.17845787778712233No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA35180.17600639586630118No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT35090.1755561236767626No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATTATAAATTATTCGGCG34170.1709533412948127No Hit
CTACTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAG33880.16950246423963286No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATTATAAATTATTCGG33710.16865195010383777No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATTCTTACTGGTTCCAGCAGAAG32690.16354886528906723No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT32210.16114741361152818No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC31400.157094963905681No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAATTTATAATTACCA31070.15544396587737286No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA30840.15429327028188541No Hit
CCTCATAGATGACCAGTAGAGGGGCCTGGCCTGACTTCTGCTGGAACCAG30500.15259224201029523No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG30000.150090729846192No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC29410.14713894549255024No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29260.14638849184331928No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAATTTATAATTACCAC29130.14573809868065246No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG29070.14543791722096008No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC28870.1444373123553188No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC28500.14258619335388242No Hit
GTCTACATGAAGTTGAGTAGCCTGAGTCCTGAAGACACGGCCGTCTATTA28460.14238607238075415No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG27980.1399846207032151No Hit
GTATCAACGCAGAGTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAA27600.13808347145849664No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26930.13473144515859836No Hit
CATGTAGACTGTCCTCGTGGACGTGTCCCTGGTCATATTGACTCTGCCCT26590.1330304168870082No Hit
ACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCC26230.13122932812885388No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25830.12922811839757134No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA25520.12767718085582733No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA25470.12742702963941704No Hit
GACTATCAGTGGGGCCCAGGTTGAGGATGAAGCTGACTACTACTGTTACT25080.12547585015141655No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT25020.12517566869172414No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24940.12477542674546763No Hit
TCCTCAACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAGCT24720.12367476139326222No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24080.12047282582321013No Hit
CTCTACTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAG23500.11757107171285042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.11752104146956835No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC22830.11421904541295212No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA22740.11376877322341356No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22650.11331850103387497No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG22600.11306834981746466No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22600.11306834981746466No Hit
GGTTGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTA22340.111767563492131No Hit
TACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCAACC22280.11146738203243861No Hit
GTCGTTTACTGTCCTCATAGATGACCAGTAGAGGGGCCTGGCCTGACTTC22110.11061686789664352No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG21870.10941614205787398No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC21730.10871571865192509No Hit
CCTTGACTATCAGTGGGGCCCAGGTTGAGGATGAAGCTGACTACTACTGT20640.10326242213418012No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20640.10326242213418012No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA20590.1030122709177698No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20570.10291221043120566No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT20510.1026120289715133No Hit
TAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGG20370.10191160556556438No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20160.10086097045664104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31850.082.962031
GGTATCA11800.081.760881
ATCAACG40050.064.722513
TCAACGC40600.063.8457224
TATCAAC41050.063.4966432
CAACGCA46000.056.037735
AACGCAG47400.054.0787966
CGCAGAG49700.051.5761578
ACGCAGA56100.045.6922457
AGACGGC22750.042.4110226
ATAGACG24550.040.181344
AATAGAC25450.039.3262373
GTAATAG27200.038.3814931
TAATAGA26400.037.9110872
GTACATG52050.036.932891
TACATGG52250.036.518672
ACATGGG52950.035.7639243
GCCCATA5600.034.713511
CATGGGG40250.033.989374
TATTGAG49750.033.867115
Produced by FastQC (version 0.11.5)