FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933271
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG63220.32701054327096407No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGGGGTGTTCGGCGG53190.27512956021168267No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC41930.21688630305839168No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC35910.1857473680616944No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATTCAGGCTG32650.16888475542228687No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32430.1677467876981551No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32320.1671778038360892No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC30770.15916030396152428No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29050.15026346539103932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.14379773968574502No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25170.13019385280180584No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24820.12838344960432344No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTACTCAACTCCATGTAGA23130.11964178845076556No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22960.11876244975484555No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22120.11441748208088778No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21700.1122449982439089No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21560.11152083696491592No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21500.11121048213106181No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19650.10164120808722626No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35700.082.521781
TCAACGC43700.066.070554
ATCAACG44000.065.620063
GGTATCA15350.063.3488731
CAACGCA45800.063.0411155
TATCAAC46900.061.869582
AACGCAG47750.060.46516
ACGCAGA69450.041.778747
CGCAGAG69450.041.675078
GCAGAGT78700.036.959769
GTACATG53800.032.266231
GTACACG8400.028.2865285
CAGAGTA60850.025.202389
TACATGG71500.024.9741442
TATTGAG24050.023.0526375
ACACGGG10750.022.1018167
GTATGTA16550.021.3261051
TAATCAG43200.021.1672745
CATGGGG33700.021.1519434
ACGGGGT11300.021.0260669
Produced by FastQC (version 0.11.5)