FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637929
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27470.4306121841145331No Hit
GTATTATTGTGTCGCTATCTATGGCTCCGAGGCTATGGCCGTCTGGGGCC21410.33561728656323825No Hit
CAATAATACACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTCCAGATA21190.3321686269161615No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20480.32103886169150486No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17680.2771468298196194No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17390.27260086937574557No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17180.26930896698535417No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16740.26241164769120073No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16680.2614711041510889No Hit
ATAATACACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTCCAGATACA15820.24798998007615267No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15730.2465791647659849No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15390.24124941803868455No Hit
ATCTATGGCTCCGAGGCTATGGCCGTCTGGGGCCAAGGAACCACGGTCAC14710.23058992458408384No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14270.2236926052899304No Hit
CCCCTACATGGACTGGGTCCGCCAGGCTCCGGGGAAGGGGCTGGAGTGGG13490.21146553926847658No Hit
GGCTTACAGTGGGGCTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAA12790.20049253130050523No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12630.19798441519354038No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12420.19469251280314895No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12210.19140061041275752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12050.18889249430579266No Hit
GAGTACGCCGCGTCTGTGAAAGGCAGATTCATCATCTCAAGAGATGATTC12050.18889249430579266No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11670.18293571855175106No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11260.17650867102765355No Hit
CTGTAAGCCTGGTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT11200.17556812748754172No Hit
GCCTGGAGGGTCCCTGAGACTCTCCTGTGCGGCCTCTGGATTCACGTTCA10730.16820053642333238No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10710.16788702190996177No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT10620.166476206599794No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC10290.16130321712917894No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10160.15926537279227No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10120.15863834376552877No Hit
GTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCTTCAAGATT10060.15769780022541693No Hit
ATACACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTCCAGATACAGTG9970.1562869849152492No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9850.15440589783502554No Hit
GTGTATTATTGTGTCGCTATCTATGGCTCCGAGGCTATGGCCGTCTGGGG9750.15283832526817248No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9540.14954642287778105No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG9530.14938966562109576No Hit
ATGTAGGGGTCACTGAACGTGAATCCAGAGGCCGCACAGGAGAGTCTCAG9500.14891939385103986No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9460.14829236482429864No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCTTCAAGATTCAGTG9360.14672479225744556No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG9320.14609776323070436No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG9110.14280586084031296No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9030.1415518027868305No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8970.1406112592467187No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8970.1406112592467187No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8930.13998423021997747No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8920.13982747296329215No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8840.1385734149098097No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8680.13606529880284482No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8630.1352815125194183No Hit
CTCCTGTGCGGCCTCTGGATTCACGTTCAGTGACCCCTACATGGACTGGG8620.13512475526273302No Hit
GCCATAGATAGCGACACAATAATACACGGCCGTGTCCTCGCTTTTCAGGC8570.13434096897930647No Hit
CTATGGCTCCGAGGCTATGGCCGTCTGGGGCCAAGGAACCACGGTCACCG8350.13089230933222976No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8300.13010852304880324No Hit
TCCAGATACAGTGAGTTCTTTGAATCATCTCTTGAGATGATGAATCTGCC8230.1290112222520061No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT8210.12869770773863548No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8170.12807067871189426No Hit
CCCTGAGACTCTCCTGTGCGGCCTCTGGATTCACGTTCAGTGACCCCTAC8090.12681662065841184No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8030.12587607711829998No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT7990.12524904809155876No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7920.12415174729476164No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7900.12383823278139103No Hit
GTCCATGTAGGGGTCACTGAACGTGAATCCAGAGGCCGCACAGGAGAGTC7880.12352471826802042No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7870.12336796101133513No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7830.1227409319845939No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7820.12258417472790858No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7780.12195714570116738No Hit
GTCTGGGGGAGGCTTGGTGCAGCCTGGAGGGTCCCTGAGACTCTCCTGTG7760.12164363118779677No Hit
CTACATGGACTGGGTCCGCCAGGCTCCGGGGAAGGGGCTGGAGTGGGTTG7650.11991930136425841No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7460.1169409134872376No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC7360.11537334092038457No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7050.1105138659631401No Hit
GCCGTGTATTATTGTGTCGCTATCTATGGCTCCGAGGCTATGGCCGTCTG7010.10988683693639889No Hit
GTATCTGGAAATGAACAGCCTGAAAAGCGAGGACACGGCCGTGTATTATT7000.10973007967971357No Hit
TACATGGACTGGGTCCGCCAGGCTCCGGGGAAGGGGCTGGAGTGGGTTGG6950.10894629339628704No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG6940.10878953613960174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6930.10863277888291643No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA6890.10800574985617521No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC6870.1076922353428046No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAT6830.1070652063160634No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT6830.1070652063160634No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA6800.10659493454600746No Hit
GGGCTGGAGTGGGTTGGTCGTATTAGAAACAAACCTAACAGTTACGCCAC6700.10502736197915442No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6670.10455709020909851No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6660.10440033295241322No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6620.10377330392567197No Hit
GTGTGAGGTGCAACTGGTGGAGTCTGGGGGAGGCTTGGTGCAGCCTGGAG6620.10377330392567197No Hit
GCCATAGCCTCGGAGCCATAGATAGCGACACAATAATACACGGCCGTGTC6610.10361654666898668No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6530.10236248861550422No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6490.10173545958876301No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC6470.1014219450753924No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC6390.10016788702190997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)