FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574153
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.22920719738466927No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA8960.15605596417679607No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG8650.15065670648764354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.1165194643239694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.10937851060605797No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5800.10101836966801532No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG5780.10067003046226354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG27550.041.026763
TCAACGC27800.040.6578144
CAACGCA28400.039.7988435
AACGCAG29650.037.8781786
TATCAAC30500.037.0585982
CGCAGAG30700.036.5826728
ACGCAGA30850.036.4047977
GCAGAGT32600.034.450559
GTATCAA40050.031.830911
TACGGGC1153.293255E-431.3012248
AATCCGC950.004890708330.3127636
ATACCGT1204.2261943E-429.9970056
ACGGGCC1306.7510334E-427.6895439
CATGGGG9200.026.606044
GTACGGA1658.823832E-526.1792056
GTACATG26850.024.4104291
TACATGG26800.023.6394312
TAGCGCG1550.001882896923.2234884
GTATATA5900.023.1942251
ACATGGG27150.022.5391863
Produced by FastQC (version 0.11.5)