FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574153
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA9380.1633710874975834No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG7320.12749214930515038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGGC855.5459328E-542.372562
TCAACGC20650.029.30174
ATCAACG20850.028.6751443
TATTACG3052.5465852E-1128.3237027
GTATAAC1555.7367797E-527.8887391
CGCAGAG22100.027.6882368
CAACGCA22500.027.2102245
ACGCAGA22800.027.1539027
AACGCAG23700.026.122746
CTAGACT1959.1954735E-625.8581244
TATCAAC23500.025.7480892
GCATCGC1400.001040991625.7105067
AGGCCGT3109.295036E-1025.5446326
GCAGAGT24550.024.925059
ATTACGG3201.3442332E-924.746368
CATGGGG10050.024.3694924
TAGACTA2101.6343194E-524.0090225
CCGTGCA3352.2846507E-923.6383139
GTCCATA2452.0169591E-623.5251941
ATACCGT1650.002708392721.8149746
Produced by FastQC (version 0.11.5)