FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637274
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGGCAGCTCACCTCTCACTTTCGGCGGAGGGA76290.46595743901143No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT74440.4546581696160814No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT44380.27106031122463314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44210.27002199998289844No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC42210.25780657360954856No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT40830.24937792941193715No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40640.24821746390646893No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT36640.22378661115976922No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32830.20051622391853777No Hit
GTGTATTACTGTCACCAGTATGGCAGCTCACCTCTCACTTTCGGCGGAGG31370.19159896266599238No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT30250.18475832389691646No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGCGATGCCAGTGGCCCTCCT30090.1837810897870485No Hit
ATCCAGGAGGGCCACTGGCATCGCAGACAGGTTCAGTGGCAGTGGGTCTG29980.18310924133651424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29980.18310924133651424No Hit
GTATGGCAGCTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA29790.18194877583104602No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA28690.17523029132570359No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28630.17486382853450308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23690.14469172539232897No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA22130.13516369282111607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21810.1332092246013801No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21560.13168229630471137No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG21430.13088829359044363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20550.1255135059861697No Hit
ATGTATTACTGTGCGAGACATTCCCAAACCTACGGCACAGACTACTGGGG20050.12245964939283224No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19840.1211770296236305No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19750.12062733543682977No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18990.11598547341495681No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA18960.11580224201935656No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC18830.11500823930508883No Hit
CTCCTGCGGGGCCAATGAGAGTGTCAGCAGCAGCTCCTTAGCCTGGTACC18830.11500823930508883No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA18740.11445854511828808No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG18400.11238192263481861No Hit
GCCATACTGGTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC18170.11097714860188337No Hit
CTCCTGGATGTATCATAGATGAGGAGCCTGGGCGCCAGGCCAGGCTTCTG18110.11061068581068288No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17740.10835083193161317No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16810.10267065866800548No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16590.101326961766937No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)