FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797550
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA76520.42569052321214984No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG57320.31887847347778925No Hit
GTGTATTACTGTGCGAAAGTAGGAAGGGAAAATGCCGACATCTGGTTCGA54990.3059163861923173No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG44630.2482823843564852No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT40680.22630803037467662No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT39730.22102305916386192No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG39230.21824149536869628No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT35580.1979360796639871No Hit
GTATAAAGCAAGATGGAAGTCAGAAAGAGTATCTGGACTCTGTGAAGGGC35460.19726850435314733No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG34260.1905927512447498No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC31610.1758504631303719No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT30000.16689382770993855No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27790.1545993157353064No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT26680.14842424411003866No Hit
GCTTTATACTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC25380.141192178242608No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC24220.13473895023782373No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT23850.13268059302940113No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23410.13023281688965535No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC22650.1260048399210036No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC22620.12583794609329366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22460.12494784567884064No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC22130.12311201357403131No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT21490.1195516119162193No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAGTG19380.1078134127006203No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT19240.10703457483797392No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19140.10647826207894077No Hit
CCCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGAGTCGAACCAGA19080.1061444744235209No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG18880.10503184890545464No Hit
GTCCAGATACTCTTTCTGACTTCCATCTTGCTTTATACTGGCCACCCACT18360.1021390225584824No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG18000.10013629662596311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC41600.048.1114354
GTATCAA42950.047.716431
CAACGCA43050.046.658195
TATCAAC43300.045.9168172
ATCAACG43650.045.686973
AACGCAG44950.044.685996
ACGCAGA49600.040.4966777
CGCAGAG51050.039.4874538
GTCATAG26950.037.2193531
ATAGGTG26600.036.5383534
TAGGTGG27750.035.8024675
TCATAGG28300.035.6362652
GCAGAGT59000.034.1666879
CAGCCGT29600.034.051269
GTAATAC31100.033.877021
CATAGGT29650.033.508213
GTGGTCA29350.033.1148268
GGTGGTC30250.032.8435867
ACAGCCG30600.032.7031978
AATACAC33200.031.87693
Produced by FastQC (version 0.11.5)