FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803500
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAAGGCGGTGTCCTCAGTTCGCAGAGTGTTCATTTGCAGATAGA14110.17560672059738644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.14735532047293093No Hit
GTATAATAACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGA11470.14275046670815184No Hit
ACCATACACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGTGGGTCTC8500.10578718108276292No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8040.10006222775357809No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16250.067.43621
TCAACGC16800.063.5000574
ATCAACG17500.060.9600563
GGTATCA7400.058.45511
CAACGCA18300.058.2951355
TATCAAC19700.054.1523362
AACGCAG19850.053.7364276
ACGCAGA21750.049.0238887
TTAGCGT803.8639628E-545.0507164
CGCAGAG27350.038.9594048
GCAGAGT30900.034.4834829
GTACATG21650.030.3030511
ACATGGG20750.029.8745963
TACATGG21950.027.9129712
CAGAGTA25150.025.4776829
TCTATAC4600.025.0717033
CAAGGCG7950.024.4681957
GTGCTAG3203.5905032E-822.529571
GTATTAG5150.022.3983291
AGGCGGT8750.022.2158859
Produced by FastQC (version 0.11.5)