FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803500
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAAGGCGGTGTCCTCAGTTCGCAGAGTGTTCATTTGCAGATAGA13380.16652146857498445No Hit
GTATAATAACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGA12410.15444928438083386No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8820.10976975731176103No Hit
ACCATACACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGTGGGTCTC8100.10080896079651525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9700.057.212434
ATCAACG10000.055.4960563
CAACGCA10400.053.3649185
GTATCAA11750.049.693521
AACGCAG11450.048.4409836
ACGCAGA12250.045.2774937
TATCAAC13350.042.6498222
TATACCG700.001090689741.1613586
ATACCGC1051.9252165E-434.301137
GGTATCA6200.031.3924141
ATACGCT1204.2140938E-430.013499
GTACATG20700.028.5559161
CGCAGAG19650.028.2264278
ATAGCCG1050.00793305127.4563053
TACATGG21050.026.3639182
ACATGGG20800.026.3342933
CAAGGCG8850.025.231687
GTAATAC9550.024.9093951
TAGACCC1851.905017E-423.374964
TACCGCA1851.9122742E-423.361858
Produced by FastQC (version 0.11.5)