FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819246
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27830.3397026045900743No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21840.2665865930379886No Hit
GTAATACACGGCCGTGTCGTCGACTCTCAGGCCGTTCATTTGCAGATACA18630.22740422290740508No Hit
ATGCTAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA16320.1992075640283871No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15060.18382756827619542No Hit
ATATTACTGTCAACACTATGCTAATCTCCCGCTCACTTTCGGCGGAGGGA14620.17845677610876343No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14410.17589344348339814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12230.1492836095629396No Hit
GTGTATTACTGTGCGAGAGAAGGGGCCCAGTTTGGCGACGACTACATTTA12170.14855122881283522No Hit
ACATATTACTGTCAACACTATGCTAATCTCCCGCTCACTTTCGGCGGAGG12060.1472085307709772No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11760.1435466270204554No Hit
AGTCAGGACATTACCACGTATTTAAATTGGTATCAGCAGCAGTCAGGAAA11610.14171567514519448No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCT11510.1404950405616872No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11470.14000678672828432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.13488012147755377No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11020.13451393110250157No Hit
GTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGG11000.1342698041858001No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT10950.13365948689404647No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG10820.13207266193548703No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAC10660.1301196466018754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10640.12987551968517394No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10560.1288990120183681No Hit
GATCTACGGTGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG10270.125359171726197No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA10130.12365028330928683No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG10130.12365028330928683No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9570.1168147296416461No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9110.11119981055751264No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9100.1110777470991619No Hit
GTCAACACTATGCTAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTG8880.10839235101544592No Hit
GTCTCATACATTACTAATAGTGGTAGTTCCATGTTCTACGCAGACTCTGT8820.10765997026534153No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8730.10656139914018499No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8660.10570695493172991No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT8440.10302155884801391No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8310.10143473388945444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG2750.070.666739
GTATCAA20150.069.061431
ATCAACG21600.062.6796653
TCAACGC21750.062.2473874
CAACGCA22650.060.0919275
TATCAAC23550.057.7954252
AACGCAG23550.057.7636766
GTGCTAG3750.055.759651
CGCAGAG27350.049.738018
GCAGAGT30700.044.5450179
CTAGAGA4950.042.1905634
TGCTAGA6300.036.5790142
AGAATAC5450.035.6575248
ACGCAGA39450.034.6649447
GTACATG25250.033.9812131
GCTAGAG5850.033.2376633
TACATGG25100.032.4210662
CAGAGTA30150.028.408199
ACACCGG1050.00798567927.419196
GGTATCA25850.025.1035581
Produced by FastQC (version 0.11.5)