FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819246
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26710.32603149725479286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19040.23240882469978494No Hit
GTAATACACGGCCGTGTCGTCGACTCTCAGGCCGTTCATTTGCAGATACA18020.21995835194801072No Hit
ATGCTAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA15930.19444708915270872No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14880.18163042602588234No Hit
ATATTACTGTCAACACTATGCTAATCTCCCGCTCACTTTCGGCGGAGGGA14050.17149915898277196No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13250.16173408231471378No Hit
GTGTATTACTGTGCGAGAGAAGGGGCCCAGTTTGGCGACGACTACATTTA12000.14647615002087286No Hit
ACATATTACTGTCAACACTATGCTAATCTCCCGCTCACTTTCGGCGGAGG11730.1431804366454032No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11360.1386640886864263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10950.13365948689404647No Hit
GTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGG10820.13207266193548703No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10660.1301196466018754No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT10450.12755631397651013No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10110.12340615639258537No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG10080.1230399660175332No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG10080.1230399660175332No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9680.1181574276835041No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9670.11803536422515337No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCT9650.11779123730845191No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAC9490.11583822197484027No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9410.11486171430803446No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9350.11412933355793009No Hit
AGTCAGGACATTACCACGTATTTAAATTGGTATCAGCAGCAGTCAGGAAA9330.11388520664122864No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9140.11156600093256482No Hit
GATCTACGGTGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG8770.1070496529735879No Hit
GTCTCATACATTACTAATAGTGGTAGTTCCATGTTCTACGCAGACTCTGT8680.10595108184843136No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA8680.10595108184843136No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8370.10216711463955881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG259.694224E-486.2955255
GTATCAA12550.060.7986641
TCAACGC12650.059.6796344
ATCAACG13100.058.174873
CAACGCA13150.057.420975
ACGCGTG400.006242212453.947897
AACGCAG14200.053.188066
TATCAAC14900.050.181992
AACGCGT450.00994349347.953686
CGCAGAG16350.045.7539678
TATACCG951.0791677E-437.8489155
GAATACG2700.037.2973069
GCAGAGT19500.036.518579
GTACATG24200.035.0993081
TACATGG24500.034.33372
GTGCTAG3850.033.6545451
ACATGGG28900.029.1064223
ACCGATG1000.00631236528.7722078
CAGAGTA18500.025.6616979
ACGCAGA29650.025.4728647
Produced by FastQC (version 0.11.5)