FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064818
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT40150.3770597416647728No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37410.35132764472426276No Hit
GTAATACACAGCCGTGTCCTCGGCTCCCAGACTGTTCATTTGCAGATGCA34800.3268164136969886No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27870.2617348692452607No Hit
GTGTATTACTGTGCAAGAGCGATAGCGGGAGCTAATATGTTGAGGGGCGG23860.2240758514600617No Hit
GCCTACAATCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21600.2028515671222688No Hit
TTATTACTGCATGCAAGGCCTACAATCTCCTCTCACTTTCGGCGGAGGGA21360.20059766082091024No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21090.19806201623188188No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA20780.19515072059262709No Hit
TATTAATAGTGATGGGAGTAGTACAAGTTACGCGGACTCCGTGAAGGGCC20090.1886707399762213No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18460.17336295967949453No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC17880.16791601945121137No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17680.16603776420007926No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17070.1603090856841263No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16360.15364127954260728No Hit
ATTGTAGGCCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACT16250.15260823915448463No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15910.14941520522756002No Hit
CTATTAATACGTGAGACCCACACCAGCCCCTTCCCTGGAGTTTGGCGGAC15060.1414326204102485No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14850.1394604523965598No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC14360.1348587270312861No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.1347648142687295No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14170.1330743845427106No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT14140.13279264625504075No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14010.1315717803418049No Hit
GTTTATTACTGCATGCAAGGCCTACAATCTCCTCTCACTTTCGGCGGAGG13420.12603092735096513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13010.1221805040861443No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG12690.11917529568433291No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11960.11231966401770069No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11370.10677881102686092No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11340.10649707273919112No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA11280.10593359616385147No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA11220.10537011958851185No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11160.10480664301317219No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11060.10386751538760614No Hit
GTCCCTGAGAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA10770.10114404527346457No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10770.10114404527346457No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10750.10095621974835135No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)