FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064818
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT36430.34212419399371535No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34260.32174512451893184No Hit
GTAATACACAGCCGTGTCCTCGGCTCCCAGACTGTTCATTTGCAGATGCA30950.2906600001126953No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26110.24520622303529807No Hit
GTGTATTACTGTGCAAGAGCGATAGCGGGAGCTAATATGTTGAGGGGCGG23000.2159993538801936No Hit
GCCTACAATCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20880.19608984821819314No Hit
TTATTACTGCATGCAAGGCCTACAATCTCCTCTCACTTTCGGCGGAGGGA20620.19364811639172141No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20240.19007943141457037No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA19760.1855716188118533No Hit
TATTAATAGTGATGGGAGTAGTACAAGTTACGCGGACTCCGTGAAGGGCC18570.17439600006761719No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC17680.16603776420007926No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17090.1604969112092395No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16840.15814909214532435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16330.15335954125493748No Hit
ATTGTAGGCCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACT15830.14866390312710717No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15270.14340478842393725No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14380.1350465525563993No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT14110.13251090796737094No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC13480.12659440392630478No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13450.12631266563863497No Hit
CTATTAATACGTGAGACCCACACCAGCCCCTTCCCTGGAGTTTGGCGGAC13280.12471614867517265No Hit
GTTTATTACTGCATGCAAGGCCTACAATCTCCTCTCACTTTCGGCGGAGG13020.12227441684870091No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12610.11842399358388006No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG12430.11673356385786114No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11550.10846924075287984No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11520.10818750246521001No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11460.1076240258898704No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11300.10612142168896468No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11280.10593359616385147No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA11190.10508838130084203No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11170.10490055577572881No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11160.10480664301317219No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10950.10283447499948348No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10790.10133187079857779No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA10790.10133187079857779No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG13550.062.8010143
TCAACGC13750.061.3630754
GTATCAA14800.060.443211
CAACGCA14400.058.6152955
GGTATCA5500.057.7135241
AACGCAG15500.054.835686
TATCAAC15950.054.707722
ACGCAGA16700.050.8977857
CGCAGAG17600.047.8857778
GTACATG26950.033.1933061
ACATGGG28050.031.8795913
TACATGG27450.031.7882732
ACAGCCG15350.027.2177948
CAGCCGT15450.027.0390859
AATACAC17000.026.3006613
GTAATAC16000.026.151441
TAATAGT17400.024.8671464
CATGGGT5700.024.038244
TAATACA17850.023.836242
TAGTGAT16100.023.2654177
Produced by FastQC (version 0.11.5)