FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623841
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC32710.5243323218576529No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA25790.41340662123842453No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTGTAG24300.3895223302091398No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG24130.38679727687022813No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19540.31322083671961287No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTATGTGGTATTCGG19260.30873251357317005No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC19060.3055265684685681No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17760.2846879252886553No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16520.2648110656401231No Hit
GTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGCAGATACA16330.2617654177907512No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16120.2583991754309191No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15850.25407114953970644No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG14870.23836201852715674No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14220.22794269693720035No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13990.2242558600669081No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13940.22345437379075755No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGCTGGTTATAACTATGTCTCCT13730.22008813143092554No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT13050.20918791807527878No Hit
GTTATAACCAGCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG12940.2074246482677477No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12510.2005318662928535No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12490.20021127178239326No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12450.1995700827614729No Hit
GTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC12380.19844800197486218No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12320.1974862184434816No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12060.19331848980749905No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11940.19139492274473785No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTGTAGC11900.19075373372381743No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT11900.19075373372381743No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11890.19059343646858734No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11740.18818897764013587No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10980.17600638624264836No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10900.17472400820080758No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10830.1736019274141969No Hit
CTTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10800.1731210356485066No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10690.1713577658409755No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG10500.16831211799160364No Hit
ATACAAGGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGCAGATACAGGG10500.16831211799160364No Hit
CCACATAAGTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCA10300.16510617288700166No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10290.16494587563177157No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10210.16366349758993076No Hit
CATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAG10130.16238111954808998No Hit
TTGTATTACTGTGCAAATGGGGGGTTTCTGACTGGTTATTATTTCTACCA9980.1599766607196385No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9860.1580530936568773No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC9390.15051912266106265No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9270.1485955555983015No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC9050.14506901598323932No Hit
GCTCATATGCAGGCAGCTACACTTATGTGGTATTCGGCGGAGGGACCAAG8910.14282485441001794No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8900.14266455715478782No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGCTGGTTATA8830.14154247636817716No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC8640.13849682851880527No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8360.1340085053723625No Hit
TCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGC8240.13208493830960133No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG8210.13160404654391103No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT8090.12968047948114986No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC8060.12919958771545956No Hit
GCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGG8060.12919958771545956No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG7970.12775691241838866No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7810.1251921563347071No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG7710.1235891837824061No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT7630.12230680574056531No Hit
GTCTGAGACCTGAGGACACGGCCTTGTATTACTGTGCAAATGGGGGGTTT7570.12134502220918471No Hit
GCCTTGTATTACTGTGCAAATGGGGGGTTTCTGACTGGTTATTATTTCTA7530.12070383318826432No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7410.11878026612550313No Hit
TCCTACCACTATTCCAACTAATACCTGAGACCCACTCCAGGCCCTTCCCT7390.11845967161504294No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7390.11845967161504294No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT7240.11605521278659145No Hit
TTATTACTGCTGCTCATATGCAGGCAGCTACACTTATGTGGTATTCGGCG7110.11397134846860017No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7060.11316986219244968No Hit
GTGGTAGGATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCC7030.11268897042675938No Hit
GTATCAACGCAGAGTACATGGGGCTGGGGTCTCAGGAGGCAGCACTCTCG7000.11220807866106908No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA7000.11220807866106908No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTT6970.11172718689537878No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG6920.1109257006192283No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG6920.1109257006192283No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT6900.1106051061087681No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT6800.10900213355646711No Hit
GTATTACTGTGCAAATGGGGGGTTTCTGACTGGTTATTATTTCTACCAGT6630.10627708021755543No Hit
GTCTCAGACTGCTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTG6550.10499470217571465No Hit
AAGTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCA6480.10387262138910394No Hit
CAGTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGCAGATA6470.10371232413387385No Hit
TTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGC6470.10371232413387385No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC6400.10259024334726317No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6340.10162845981588257No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC6290.10082697353973208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3850.074.962661
GTATCAA9550.068.751751
ATCAACG11200.057.297863
TATCAAC11600.055.322072
TCAACGC11700.054.8448224
CAACGCA12200.052.010295
AACGCAG12750.049.6987956
ATAGCGT450.0099008648.004526
GTCGTGC450.0099008648.004528
ACGCAGA13450.047.647617
CGCAGAG13650.046.949488
GCAGAGT14300.044.8118139
ATAGGAC951.0620464E-437.950093
GCCCATA1406.858081E-736.0757831
GTCTACA1001.4308192E-436.0757831
ATTGAGG5100.035.2974436
CTAAGGG1051.9133327E-434.3357963
ATGGGTA850.002815568633.9318435
GTGCTAG2353.6379788E-1133.773071
TATTGAG11500.033.231085
Produced by FastQC (version 0.11.5)