FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782037
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAGGTCCAAC25760.3293961794646545No Hit
CTACTATACTGTTGGCAGTAGTAAGTTCCAAAATCATCAGGCTGCAGGCT25240.32274687770527477No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21910.2801657722077089No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16820.21507933767839627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.20421028672556416No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCCTGACTACTGGGGCTGGGTCCG14850.18988871370536178No Hit
ACTATACTGTTGGCAGTAGTAAGTTCCAAAATCATCAGGCTGCAGGCTGC13010.16636041517217215No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12150.15536349303165964No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCACTGA10940.13989107932233386No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10700.13682217081800477No Hit
TTCTAAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTC10570.13515984537815987No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10550.1349041030027991No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10490.13413687587671683No Hit
GAGTATCACTGAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAGAGCCC10480.13400900468903645No Hit
CAGTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAGGTCCA10330.13209093687383078No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10150.12978925549558396No Hit
GTAGTAGGTGCTCCCAGGAGAATAGATACTCGCAATCCATTCCAGCCCCT9670.12365143848692582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9600.12275634017316316No Hit
GAATTCACACTCACCATCAGCAGCCTGCAGCCTGATGATTTTGGAACTTA9350.11955956048115371No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9320.11917594691811258No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9240.11815297741666954No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9020.11533981128770121No Hit
GGTATCAGCAGAAGCCAGGGAGAGCCCCTAAGGTCCTGATCTATAAGACG8670.110864319718888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.10626095696239436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8250.1054937298363121No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8240.10536585864863171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.1019133365812615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.072.096051
ATCAACG22550.063.9555633
TCAACGC22650.063.354834
CAACGCA23400.061.3242265
TATCAAC24350.059.523982
AACGCAG24450.058.6869136
ACGCAGA27000.053.103457
CGCAGAG27500.052.114598
GCAGAGT30400.047.1280639
GTGCTAG3500.041.197741
GTACGGA750.001523513338.456156
GAATACG3500.037.0258069
CTAGAGA4550.031.696664
TGCTAGA5250.030.217482
GGTATCA23800.029.989531
GCTAGAG4100.029.8992273
GTGTAGA4250.028.8384171
GTACATG21200.027.8862061
TACATGG21550.027.7731842
GATTCAC2551.0007534E-725.4456631
Produced by FastQC (version 0.11.5)