FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782037
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAGGTCCAAC24480.3130286674415661No Hit
CTACTATACTGTTGGCAGTAGTAAGTTCCAAAATCATCAGGCTGCAGGCT23570.30139238936265167No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21690.2773526060787405No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15930.20369880197484264No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCCTGACTACTGGGGCTGGGTCCG13880.1774852085003651No Hit
ACTATACTGTTGGCAGTAGTAAGTTCCAAAATCATCAGGCTGCAGGCTGC12040.15395690996717548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11870.15178309977660903No Hit
GAGTATCACTGAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAGAGCCC10700.13682217081800477No Hit
TTCTAAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTC10580.13528771656584024No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCACTGA10530.13464836062743835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9900.12659247580357452No Hit
CAGTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAGGTCCA9640.12326782492388466No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9480.12122188592099863No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9470.12109401473331825No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9230.11802510622898915No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9000.11508406891234046No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8950.11444471297393857No Hit
GTAGTAGGTGCTCCCAGGAGAATAGATACTCGCAATCCATTCCAGCCCCT8800.1125266451587329No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8780.11227090278337214No Hit
GAATTCACACTCACCATCAGCAGCCTGCAGCCTGATGATTTTGGAACTTA8770.11214303159569176No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8320.10638882815007475No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA8200.10485437389791019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.060.2601241
TCAACGC13250.056.0665444
ATCAACG13400.055.4424933
CAACGCA14300.051.959775
TATCAAC14600.051.3796122
AACGCAG14600.050.3398636
GTATACG450.00983065448.0922161
CGTATAC901.3708068E-648.0860443
ACGCAGA16000.045.9351237
GAATACG2900.044.723859
CGCAGAG16700.044.0096978
GTGCTAG3450.039.728351
GCAGAGT18850.038.9900259
CACTAGT1153.258005E-431.3604642
GTACATG23200.030.7831631
TACCGTC950.004871128630.338997
GCTAGAG4400.029.5073453
TACATGG23850.029.3355122
CATTAGC6050.028.5838388
CTAGAGA4900.027.966624
Produced by FastQC (version 0.11.5)