FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120362
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44760.3995137285984352No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATG33970.3032055710564978No Hit
GTAATATACGGCCGTGTCGTCAGATCTCAGGGTGCGCAGCTCCATGTAGA33840.3020452318090046No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT30800.27491114479070156No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGG30720.2741970898691673No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29460.2629507248550022No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26650.2378695457361103No Hit
GTATATTACTGTGCGAGAGCCCACAGCGGCGGTTATGATTTGTGGACTGG25690.22930088667769882No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22810.2035949095024644No Hit
CTCCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC21890.19538327790482005No Hit
TCCAAGAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT21350.1905634071844636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19830.17699636367531207No Hit
TTATTACTGCATGCAAGCTCTACAAACTTGGACGTTCGGCCAAGGGACCA19300.17226574982014742No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT18340.16369709076173594No Hit
ACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC17540.15655654154639306No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17410.1553962022988998No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16990.1516474139608448No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA16740.14941599233105016No Hit
GTCTACATGGAGCTGCGCACCCTGAGATCTGACGACACGGCCGTATATTA16370.14611348831895407No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA16170.14432835101511832No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15770.14075807640744686No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15640.13959773715995366No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA15530.138615911642844No Hit
CCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG15500.13834814104726864No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT15330.13683077433900828No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15210.13575969195670684No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15010.13397455465287111No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14950.1334390134617204No Hit
GCCGTATATTACTGTGCGAGAGCCCACAGCGGCGGTTATGATTTGTGGAC14920.13317124286614504No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14730.1314753624275011No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14470.12915468393251467No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14220.12692326230272No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14170.12647697797676108No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14060.12549515245965143No Hit
ATATACGGCCGTGTCGTCAGATCTCAGGGTGCGCAGCTCCATGTAGACTA14010.12504886813369248No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA13620.12156785039121283No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT13500.1204967680089114No Hit
CCGTAGTAGTACTCGTACCTAGGGCCAGTCCACAAATCATAACCGCCGCT13310.11880088757026747No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTTGGCCGAACGTC12930.11540912669297959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.1141595305802946No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12700.11335621879356851No Hit
GGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12630.11273142073722602No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA12600.11246365014165065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12240.10925040299474634No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT12210.10898263239917098No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATACAACTATTTGGATTGG11990.10701898136495169No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11940.10657269703899276No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11610.1036272204876638No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA11590.10344870675728024No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTTGGACGTTCGGCCAAGGGAC11400.1017528263186363No Hit
CTACAAACTTGGACGTTCGGCCAAGGGACCAGGGTGGACATCAAACGAAC11370.10148505572306094No Hit
ATATACACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGA11340.10121728512748557No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11340.10121728512748557No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11250.10041397334075951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22900.085.074261
GGTATCA6700.078.617271
ATCAACG26500.072.317853
TCAACGC26600.072.0459754
TATCAAC28050.068.321672
AACGCAG29450.065.006986
CGCAGAG33100.058.0533878
ACGCAGA35950.053.250917
GAATACG2200.049.069649
GTGCTAG3650.037.560441
CAACGCA51250.037.5325165
CAGCGCT5150.036.3337639
GTACATG30450.036.2555121
GTATACG800.002084880136.077791
ACATGGG31300.035.217363
ATAGGCG1051.9235667E-434.3076063
ATTAGAC2151.9250365E-830.1587813
GCAGCGC6050.029.7391768
CATGGGG19500.029.5573224
GTCCGAC1000.00621773428.862231
Produced by FastQC (version 0.11.5)