FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120362
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT41140.36720274339900855No Hit
GTAATATACGGCCGTGTCGTCAGATCTCAGGGTGCGCAGCTCCATGTAGA33330.2974931316842235No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATG29680.2649143758892215No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT28120.25099030491930285No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGG27350.24411752629953531No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26200.2338529868024799No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23930.21359167840394444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19370.17289054787648991No Hit
CTCCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC18950.1691417595384349No Hit
TTATTACTGCATGCAAGCTCTACAAACTTGGACGTTCGGCCAAGGGACCA18440.1645896594136538No Hit
GTATATTACTGTGCGAGAGCCCACAGCGGCGGTTATGATTTGTGGACTGG17500.1561995140856259No Hit
TCCAAGAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT17400.15530694543370804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17280.1542358630514066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16830.1502193041177762No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA15350.13700928806939186No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15310.1366522606086247No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15280.13638449001304934No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA15150.13522415076555613No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14880.1328142154053779No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA14750.13165387615788468No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14550.12986873885404898No Hit
ACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC14500.12942245452809004No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14370.1282621152805968No Hit
ATATACGGCCGTGTCGTCAGATCTCAGGGTGCGCAGCTCCATGTAGACTA14310.1277265740894461No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13890.12397778575139107No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT13370.11933642876141817No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13310.11880088757026747No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13260.11835460324430852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13250.11826534637911675No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12920.1153198698277878No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT12900.11514135609740424No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA12850.1146950717714453No Hit
CCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG12760.11389175998471922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12700.11335621879356851No Hit
GTCTACATGGAGCTGCGCACCCTGAGATCTGACGACACGGCCGTATATTA12680.11317770506318495No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12610.11255290700684242No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTTGGCCGAACGTC12340.1101429716466642No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12270.1095181735903217No Hit
CCGTAGTAGTACTCGTACCTAGGGCCAGTCCACAAATCATAACCGCCGCT12080.10782229315167775No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTTGGACGTTCGGCCAAGGGAC11820.1055016146566913No Hit
GTGTATATAGTAGCTGGTGAAGGTGTATCCAGATGCCCTGCAGGAAACCT11760.1049660734655406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11360.10139579885786916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16700.066.323991
ATCAACG17700.059.6897433
TCAACGC17900.058.629164
TATCAAC19600.055.003562
AACGCAG19150.054.4536176
GGTATCA6450.052.4088331
CGCAGAG23300.043.828838
ACGCAGA25800.039.5818567
GTATAGC1407.0374153E-735.961381
GTACATG32950.031.8686581
ACATGGG31900.031.5422693
GAATACG1902.2294262E-730.2805589
CAGCGCT4850.029.6562189
AGCGCTG4950.029.0480065
ATACGGC19900.028.9110856
TATACGG20150.028.9002425
TACGGCC19950.028.4781447
GTACACG8250.027.8860865
AATATAC23550.026.8563753
GTAATAT23000.026.580151
Produced by FastQC (version 0.11.5)