FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038899
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT52070.5012036781246301No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42970.4136109477437171No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT36070.3471944818504975No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC34990.33679886110199353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32240.310328530492377No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28100.27047865095644524No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT26110.25132375716985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25720.247569783010668No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25690.24728101576765404No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGTGGC24640.2371741622621641No Hit
TTATTACTGTCAGCAGCGTAGTGGCGACTGGCGGACTTTTGGCCAGGGGA24610.23688539501915007No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATGCCAGTGGCCCTGCT24480.23563407029942277No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24470.23553781455175143No Hit
TCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGCCCGGTTCAGTGGC24300.23390146684133875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22970.2210994524010515No Hit
GTGTTAGTACCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCC21570.20762364772706493No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20280.19520665627746298No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT19670.18933505566951167No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA19060.18346345506156037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.17807313319196572No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18140.17460792627579774No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17690.17027641763058776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC17610.1695063716492171No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTA17560.16902509291086043No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTATCAAC17490.1683513026771611No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16770.16142088884482514No Hit
CAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGT16600.15978454113441248No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16550.15930326239605583No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGGC16300.1568968687042725No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16160.15554928823687386No Hit
GTTTATTACTGTCAGCAGCGTAGTGGCGACTGGCGGACTTTTGGCCAGGG15740.15150654683467785No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC15660.15073650085330723No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA15390.1481375956661812No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC15350.14775257267549588No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15140.1457312019743979No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15020.1445761330023419No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14680.1413034375815166No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14650.1410146703385026No Hit
CCTCATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGCCCGGTTCA14530.1398596013664466No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14470.1392820668804186No Hit
TACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT14390.13851202089904793No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14320.13783823066534862No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14080.13552809272123661No Hit
CATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGCCCGGTTCAGTG14060.13533558122589395No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC13640.13129283982369797No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13390.12888644613191466No Hit
GAGTGTTAGTACCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTC12860.123784891505334No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12710.12234105529026403No Hit
CACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGC12710.12234105529026403No Hit
GTCAGAGTGTTAGTACCTACTTAGCCTGGTATCAACAGAAACCTGGCCAG12650.12176352080423601No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12580.1210897305705367No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12400.1193571271124527No Hit
ACTGTCAGCAGCGTAGTGGCGACTGGCGGACTTTTGGCCAGGGGACCAAG12370.11906835986943871No Hit
GCGTAGTGGCGACTGGCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCA12140.11685447767299806No Hit
GGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCA12080.11627694318697004No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC12080.11627694318697004No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG11610.11175292304641742No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11570.11136790005573206No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11560.11127164430806075No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11450.11021283108367608No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGT11450.11021283108367608No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCGGGCTGGGATGCCA11370.10944278510230541No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT11350.10925027360696275No Hit
ACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC11160.10742141440120744No Hit
TAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGGCTAA11150.10732515865353609No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11140.10722890290586476No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA11090.10674762416750809No Hit
GTTAGTACCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAG10940.1053037879524381No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10820.10414871898038211No Hit
GTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGG10740.10337867299901145No Hit
GGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC10730.10328241725134013No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC10560.10164606954092748No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10470.10077976781188547No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10450.1005872563165428No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAGCA10440.10049100056887146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG30450.060.347853
TCAACGC30600.060.052034
CAACGCA31850.057.69525
AACGCAG33600.054.894136
ACGCAGA37250.049.5033237
CGCAGAG40850.045.1190078
GCAGAGT40950.045.0088239
GAATACG4150.043.3714079
GTGCTAG5050.037.1050261
CCGCCGA800.002107513435.998279
TCTACAC6600.032.7556533
GTATAGT2757.2759576E-1231.4484581
TGCTAGA6250.031.1309742
GCTAGAG6100.030.7151373
GTATCAA63550.030.0525341
TATCAAC67250.027.8605692
CTAGAGA7150.027.2123874
GTACATG32450.026.6512341
TAGGACC23800.026.3421483
CCGCGGT1658.827476E-526.1805579
Produced by FastQC (version 0.11.5)