FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038899
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT48870.4704018388698035No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39420.379440157320394No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT33550.32293803343732164No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC32430.31215738969813234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31400.30224304768798504No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27020.26008303020794127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26460.2546927083383466No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATGCCAGTGGCCCTGCT25410.2445858548328567No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT25250.24304576287011537No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25210.24266073987943004No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGTGGC23510.22629726277530346No Hit
TCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGCCCGGTTCAGTGGC22150.21320648109200221No Hit
TTATTACTGTCAGCAGCGTAGTGGCGACTGGCGGACTTTTGGCCAGGGGA22130.21301396959665952No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21270.20473597529692494No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG20740.19963442067034426No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT19610.18875752118348366No Hit
GTGTTAGTACCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCC19420.18692866197772834No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC18460.1776881102012804No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18380.17691806421990972No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16980.16344225954592315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16620.15997705262975515No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16620.15997705262975515No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTATCAAC16480.1586294721623565No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTA16480.1586294721623565No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16180.15574179973221652No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA16170.15564554398454516No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC16080.15477924225550319No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGGC15950.15352791753577585No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA15880.15285412730207654No Hit
GTTTATTACTGTCAGCAGCGTAGTGGCGACTGGCGGACTTTTGGCCAGGG15660.15073650085330723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15560.1497739433765939No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15520.14938892038590854No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC15070.14505741174069856No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15010.14447987725467057No Hit
CAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGT14880.14322855253494327No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14800.1424585065535726No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14660.14111092608617393No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14200.13668316169329262No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13480.12975274786095664No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13050.12561375071108932No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12970.12484370472971867No Hit
CCTCATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGCCCGGTTCA12970.12484370472971867No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12460.11993466159848071No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG12400.1193571271124527No Hit
CATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGCCCGGTTCAGTG12340.11877959262642471No Hit
GCGTAGTGGCGACTGGCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCA12260.11800954664505404No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12260.11800954664505404No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12260.11800954664505404No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12250.1179132908973827No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12150.11695073342066938No Hit
TACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT12100.1164694546823127No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC12010.11560315295327073No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11910.1146405954765574No Hit
CACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGC11650.11213794603710274No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11590.11156041155107475No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCGGGCTGGGATGCCA11400.10973155234531942No Hit
GTCAGAGTGTTAGTACCTACTTAGCCTGGTATCAACAGAAACCTGGCCAG11350.10925027360696275No Hit
GGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCA11350.10925027360696275No Hit
ACTGTCAGCAGCGTAGTGGCGACTGGCGGACTTTTGGCCAGGGGACCAAG11260.10838397187792076No Hit
GGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC11120.1070363914105221No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG11110.10694013566285078No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11080.10665136841983677No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA11000.10588132243846611No Hit
GAGTGTTAGTACCTACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTC10910.10501502070942412No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA10730.10328241725134013No Hit
TAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGGCTAA10580.10183858103627014No Hit
GTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGG10500.10106853505489946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG18400.055.643383
TCAACGC18550.055.1934284
CAACGCA21550.047.18675
ACGCAGA22750.044.958317
AACGCAG23200.044.0862736
CGCAGAG24650.041.200768
GCAGAGT25700.039.521279
GATTCAC4600.036.0715331
GAATACG3151.8189894E-1229.7288239
GTGCTAG4400.029.513071
GTACATG30950.029.136941
TACATGG31800.027.8895782
CTCCGTT1306.7315175E-427.7058569
TCCGCTA2354.578942E-827.5852978
ACATTAG11250.025.6100527
ACATGGG33950.025.4849873
CTAGTAC1701.07070824E-425.447513
AATCCGC2301.1677566E-625.0533166
TATGGAC4350.024.862513
CATGGGG19900.024.2753074
Produced by FastQC (version 0.11.5)