FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549573
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA15640.10093103067748341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG22600.047.8813973
GTATCAA23400.047.8122181
TCAACGC23550.045.94694
TATCAAC26450.042.5497442
CGCAGAG27450.039.3514028
ACGCAGA28450.037.9682277
GGTATCA13600.031.8445721
CAACGCA36050.030.0123275
TCCGCTA8050.029.5208848
AACGCAG37050.029.1532066
TATCGTG1255.362496E-428.8052277
AATCCGC8250.028.8033686
CCGCTAC8550.028.635859
AAATCCG8500.028.0032655
GCAGAGT39250.027.7034979
GTACATG39250.027.4012051
CCGATAT1100.00990986426.2332023
TACATGG41450.025.585322
ATCCGCT9700.025.2416957
ACATGGG41150.024.7191333
Produced by FastQC (version 0.11.5)