FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083728
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGGGTAGGTCTTCT68460.32854576029117044No Hit
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGGTCACCGTCAGGGAGA67720.3249944330546021No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35420.16998379826925586No Hit
CTTATAATGGGTTCACCTACTACAGCCCGTCCCTCAGGGGTCGAGTCACC35140.16864005282839217No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32640.1566423256778236No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31970.1534269348014712No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31200.1497316348390961No Hit
GTATTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGGGTAGGTCTTCTTT31070.1491077530272665No Hit
GGATAGGGAGTATCTCTTATAATGGGTTCACCTACTACAGCCCGTCCCTC28270.1356702986186297No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27930.13403860772615236No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27590.13240691683367503No Hit
ACCTACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTCGAAGACAC27390.13144709866162954No Hit
ACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTCGAAGACACGTCC27230.13067924412399315No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25370.12175293512397012No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24720.11863352606482228No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24260.11642594426911766No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGGTCACCGTCAGGGA24020.11527416246266307No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23360.11210676249491297No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23320.11191479886050386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22630.10860342616694693No Hit
GTAGTAGGTGAACCCATTATAAGAGATACTCCCTATCCACTCCAGCCCCA22370.10735566254328781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33650.074.0614551
TCAACGC39500.061.613364
ATCAACG41250.058.999463
CAACGCA41450.058.7119675
AACGCAG43750.055.6253976
TATCAAC45550.053.902742
GGTATCA18050.052.67421
CGCAGAG52400.046.4429558
ACGCAGA59100.041.177857
GCAGAGT59800.040.575439
GTACATG54850.029.1526181
ACATGGG52650.028.1724193
TACACGG15800.027.7975926
GTACACG15400.027.584545
TACATGG58550.026.562672
ATAGCCG37550.025.8856038
TAGCCGT39350.024.701519
GTAATAC41850.024.095381
GTATGTA21250.024.0658191
CTGTGCG47500.023.9495839
Produced by FastQC (version 0.11.5)