FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083728
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGGTCACCGTCAGGGAGA64200.30810163322660156No Hit
ATGTATTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGGGTAGGTCTTCT59280.28449010619428255No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34000.1631690892477329No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31970.1534269348014712No Hit
CTTATAATGGGTTCACCTACTACAGCCCGTCCCTCAGGGGTCGAGTCACC30770.14766802576919827No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27330.1311591532100159No Hit
GTATTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGGGTAGGTCTTCTTT26590.12760782597344758No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26330.12636006234978844No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25810.1238645351024702No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25460.12218485330139058No Hit
GGATAGGGAGTATCTCTTATAATGGGTTCACCTACTACAGCCCGTCCCTC24770.11887348060783366No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23660.11354648975298118No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23580.11316256248416301No Hit
ACCTACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTCGAAGACAC22940.11009114433361744No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22420.10759561708629918No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGGTCACCGTCAGGGA22250.10677977164006051No Hit
ACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTCGAAGACACGTCC21850.10486013529596952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21070.10111684442499214No Hit
GTAGTAGGTGAACCCATTATAAGAGATACTCCCTATCCACTCCAGCCCCA20940.10049296261316257No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31650.074.845811
TCAACGC37800.061.52714
ATCAACG38250.061.1797453
GGTATCA16000.060.751791
CAACGCA41300.056.3129435
TATCAAC42100.055.5849272
AACGCAG43350.053.982136
CGCAGAG51200.045.705578
ACGCAGA56600.041.4721837
GCAGAGT60800.038.1290479
GTACATG53400.032.6301771
ACATGGG52500.031.1330933
TACATGG54700.030.802382
CAGCGCT13150.029.5645739
TACGTCG2904.292815E-1027.3118065
ATAGCCG37200.025.3561948
GTAATAC41100.023.825521
TAGCCGT41050.023.3260969
TACATAG42750.023.243355
GTACACG17200.023.0244885
Produced by FastQC (version 0.11.5)