FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387820
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGAGCAGGCTGAGATCTGAAGACACGGCTGTCTATTA46950.19662286101967485No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGCCTGCTCAGCTCCATGTAGA45190.18925212118166362No Hit
CCTTAAGACCTATCCTCCCACGTTCGGCCAAGGGACCAAGGTGGAAATCA43360.18158822691827692No Hit
GTCTATTACTGCGCGAGAGATCGCTACTTTGGTTCGGGAGATTATTACAG42800.17924299151527334No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT39210.16420835741387543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.1242555971555645No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA28860.1208633816619343No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28830.12073774405105912No Hit
GTCTTAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT26940.11282257456592207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45000.071.068721
TCAACGC48150.064.302164
CAACGCA48900.063.315935
ATCAACG49300.062.8022083
AACGCAG51450.060.4539226
TATCAAC56150.055.5253832
ACGCAGA60150.051.5902677
CGCAGAG63450.048.907088
GCAGAGT75450.041.224059
GGTATCA39100.032.9749641
GAATACG7500.032.639529
GTACATG70400.032.6381871
GTGCTAG10100.028.526381
TAGACCG1555.767382E-527.8723095
AATAGAC25850.026.461623
ACATGGG80200.026.3055063
ACAGCCG28550.025.9751018
GTAATAG26150.025.8919141
CAGCCGT29050.025.775879
TAGACAG29050.025.0339095
Produced by FastQC (version 0.11.5)