FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711284
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.1403624413013854No Hit
GCCTATATGGAATTGAAGAGACTGACATCTGAGGACACGGCCGTGTATTA22020.12867531046863057No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32500.070.709281
ATCAACG35500.063.8866543
TCAACGC36200.062.6512764
CAACGCA37650.060.2384075
AACGCAG39300.057.892526
TATCAAC40050.056.808392
GGTATCA15700.053.6852871
CGCAGAG46850.048.562998
ACGCAGA47250.048.1518757
GCAGAGT65900.034.9616939
GTACATG49600.029.9195181
ACATGGG48650.028.8588943
TACATGG52250.027.6973152
GTATAGG6900.024.0130791
CAGAGTA53150.023.7062429
CATGGGA26500.023.6374864
CAATTCG2252.7988675E-522.3997659
GTATGTA16450.022.334351
GTACACG11050.020.850465
TATACTG8750.020.5712135
Produced by FastQC (version 0.11.5)