FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097468
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT56220.26803746231170156No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA55830.2661780775678103No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGC52800.2517320884037325No Hit
GTAATACACGGCCGTGTCCGCAGATCTTAGGCTGCTCAGTTCCATGTAGG50750.24195839936533No Hit
CCTGAAGACTGTGCAACTTACTACTGTCTACAGACTTACACTACCCCTCG40760.19432954400257835No Hit
GTGTATTACTGTGCGAGGGACTGTAGTAGTTCCCGGTGCTATTGGGACTG38540.18374535392196686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37710.17978820177471122No Hit
GTAGTAAGTTGCACAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA36120.17220763320346244No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35390.16872724637515327No Hit
GACTTACACTACCCCTCGAGCGTTCGGCCAGGGGACCAAGGTGGACATCA34280.16343515133484754No Hit
GTGTAAGTCTGTAGACAGTAGTAAGTTGCACAGTCTTCAGGTTGCAGACT31880.15199278368013244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31670.15099157651034487No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA30400.14493665695972477No Hit
ACTTACTACTGTCTACAGACTTACACTACCCCTCGAGCGTTCGGCCAGGG29640.14131324053573166No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29640.14131324053573166No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28820.13740376492037065No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28700.1368316465376349No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26400.12586604420186626No Hit
GCCTAAGATCTGCGGACACGGCCGTGTATTACTGTGCGAGGGACTGTAGT26050.12419736558555362No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25330.1207646552891391No Hit
GTCTACAGACTTACACTACCCCTCGAGCGTTCGGCCAGGGGACCAAGGTG24980.11909597667282648No Hit
ACATGGAACTGAGCAGCCTAAGATCTGCGGACACGGCCGTGTATTACTGT24680.1176656807159871No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24200.11537720718504407No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23610.11256429180325991No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT23060.10994208254905438No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22560.10755825595432206No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22220.10593725386990409No Hit
TCTGTAGACAGTAGTAAGTTGCACAGTCTTCAGGTTGCAGACTGCTGATG22200.1058419008061148No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21740.10364878033896106No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21510.1025522201053842No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21480.10240919050970028No Hit
GTTCCATGTAGGCTGTATTCGTGGATTCGTCCGCGGTAATTGTGACTCGG21080.10050212923391443No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20980.10002536391496795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32600.070.899181
TCAACGC37400.060.6417924
ATCAACG38700.058.7907753
TATCAAC40300.056.9926342
CAACGCA42650.053.514735
AACGCAG44900.050.6726766
CGCAGAG48200.047.352768
GCAGAGT55500.040.8600469
ACGCAGA56600.040.197937
GTACATG51400.034.1806641
ACATGGG52150.032.306853
GAATACG3700.031.1314669
CAGCGCT11550.028.0486439
GTACACG15450.026.563145
GGTATCA52050.026.2837091
TATGTAC18150.024.991772
CATGGGA26550.024.6784
CATGGGG35000.024.2743194
GTATGTA21100.023.5461771
TGTACCG2151.9696658E-523.441895
Produced by FastQC (version 0.11.5)