FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784044
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTATCAGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA54340.6930733479243512No Hit
ATTTATTACTGTGCGAGACATGATCATACGGACCACGGTGCCCCCAACTG47470.6054507145007169No Hit
GTCTCTGGTGACTCCATCAGCAGTGGTGGTTCGTACTGGGGCTGGATCCG38610.4924468524725653No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA21690.2766426374030029No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20350.2595517598502125No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18350.23404298738336116No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT16090.20521807449581914No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC15280.19488702164674432No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT13820.17626561774594282No Hit
GTATAAAACTTATTGGACGTTCGGCCCGGGCACCAAGGTGGAGATCAAAC13480.17192912642657812No Hit
CAGTAATAAATAGCCGTATCAGCGGCGGTCACAGAGCTCAGCCTCAGGGA12660.16147052971516906No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12550.16006754722949224No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12480.15917474019315242No Hit
TTATTACTGTGCGAGACATGATCATACGGACCACGGTGCCCCCAACTGGT11980.15279754707643958No Hit
TCTCTAGGGTGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC11690.14909877506874614No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG11690.14909877506874614No Hit
CACTTGCACTGTCTCTGGTGACTCCATCAGCAGTGGTGGTTCGTACTGGG11610.14807842417007208No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11320.14437965216237864No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTGGACATGTGG11110.14170123105335927No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA10330.1317528097912872No Hit
CCCTAGAGATCAGAACCTTTGGGGCTTTCCCTGGCTTTTGCTGATACCAG10170.12971210799393912No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC10090.12869175709526506No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10030.12792649392125954No Hit
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCC9790.12486544122523736No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC9790.12486544122523736No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT9710.1238450903265633No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA9570.12205947625388372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.12193193239154947No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9440.12040140604353837No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT9410.12001877445653561No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT9030.11517210768783384No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG9000.11478947610083108No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8840.11274877430348298No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT8650.11032544091913209No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA8590.10956017774512655No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG8510.1085398268464525No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8480.10815719525944974No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAAC8300.1058614057374331No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTT8210.10471351097642478No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8200.10458596711409053No Hit
GTATGATCATGTCTCGCACAGTAATAAATAGCCGTATCAGCGGCGGTCAC8160.1040757916647535No Hit
GTAGTAGGTGTTCCCACCAGAGTCGATACTTCCAATCCACTCCAGCCCCT8130.10369316007775074No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8050.10267280917907667No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG7990.10190754600507114No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7890.10063210738172858No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)