FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784044
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTATCAGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA54790.6988128217293927No Hit
ATTTATTACTGTGCGAGACATGATCATACGGACCACGGTGCCCCCAACTG46580.594099310752968No Hit
GTCTCTGGTGACTCCATCAGCAGTGGTGGTTCGTACTGGGGCTGGATCCG36990.47178474677441573No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA20710.26414333889424574No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20210.25776614577753293No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19950.2544500053568422No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT16140.20585579380749042No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC14720.18774456535602593No Hit
GTATAAAACTTATTGGACGTTCGGCCCGGGCACCAAGGTGGAGATCAAAC13320.16988842462922998No Hit
CAGTAATAAATAGCCGTATCAGCGGCGGTCACAGAGCTCAGCCTCAGGGA13000.16580702103453376No Hit
TTATTACTGTGCGAGACATGATCATACGGACCACGGTGCCCCCAACTGGT12210.15573105591012748No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12160.1550933365984562No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11830.15088438914142574No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA11330.1445071960247129No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11260.1436143889883731No Hit
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCC11120.1418287749156935No Hit
CACTTGCACTGTCTCTGGTGACTCCATCAGCAGTGGTGGTTCGTACTGGG11000.14029824856768242No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG10910.13915035380667412No Hit
TCTCTAGGGTGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC10890.1388952660820056No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10880.13876772221967135No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10740.13698210814699174No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT10680.1362168449729862No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTGGACATGTGG10280.13111509047961592No Hit
CCCTAGAGATCAGAACCTTTGGGGCTTTCCCTGGCTTTTGCTGATACCAG10090.12869175709526506No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC10010.127671406196591No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9750.12435526577590032No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9700.12371754646422904No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT9450.12052894990587262No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC9060.11555473927483662No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA8870.11313140589048573No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG8830.1126212304411487No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8480.10815719525944974No Hit
GTATGATCATGTCTCGCACAGTAATAAATAGCCGTATCAGCGGCGGTCAC8290.10573386187509885No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8250.10522368642576181No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT8220.10484105483875907No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA8180.10433087938942202No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT8070.10292789690374521No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG7990.10190754600507114No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7970.10165245828040263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12500.046.736564
ATCAACG12950.045.112513
TATCAAC14500.041.7823682
CAACGCA14150.041.2999235
AACGCAG14800.039.4356086
CTGTGCG19850.038.8408289
GTACACG8400.037.7914925
ACGCAGA15700.037.1749657
CGCAGAG16150.036.139138
ATAGCCG19200.036.027598
TACACGG8800.036.027596
TACTGTG22450.034.6635177
TAGCCGT20000.034.5864879
ACTGTGC22900.034.297018
TTACTGT22800.034.1314056
ACACGGG9650.032.854187
AAATAGC21750.032.797536
TATGTAC9750.032.5484122
GTATGTA9800.032.3885651
GCAGAGT18050.032.335019
Produced by FastQC (version 0.11.5)