FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701543
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20490.2920704789300157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16440.23434058924399503No Hit
TTATTACTGCATGCAAAGTAAACAGCTTCCTCTCACTTTCGGCGGAGGGA15020.21409949211951368No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12250.17461509843302547No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGTAGATACA12000.17105152499561682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11640.1659199792457483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11390.16235640580833963No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11250.16036080468339076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.15337620074606972No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10490.14952754143366837Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9870.14068987930889482No Hit
GTGCAGATATTGTGATGACCCAGATTCCACTCTCTCTGTCCGTCACCCCT9570.1364135911840044No Hit
GTAAACAGCTTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAA9070.12928644430918704No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG8780.12515269912179294No Hit
ATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACT8540.12173166862188063No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8280.11802555224697558No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8280.11802555224697558No Hit
ATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAG8280.11802555224697558No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8260.1177404663719829No Hit
GTTTATTACTGCATGCAAAGTAAACAGCTTCCTCTCACTTTCGGCGGAGG8130.11588740818453039No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8120.11574486524703403Clontech Universal Primer Mix Long (96% over 26bp)
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8070.11503215055955231No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7830.11161112005963997No Hit
GTGATGGAAAAACCTATTTGTATTGGTACCTGCAGAAGTCAGGCCAGCCT7790.11104094830965458No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7470.10647957430977145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7200.10263091499737008No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7200.10263091499737008Clontech Universal Primer Mix Long (96% over 26bp)
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7190.10248837205987374No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7060.10063531387242122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)