FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523756
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG12170.23236010661453044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.16706252529803955No Hit
GTGTATTACTGTGCGAGAGATTCCGATTTTTGGAGTGGTAGAAATGATTG8390.16018909568577736No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7580.1447238790581874No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6940.13250444863638794No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6750.1288768052299162No Hit
GCATAATAGTTACCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA6650.12696751922651006No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA6590.12582194762446636No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6570.12544009042378512No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6150.11742108920947923No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6070.11589366040675429No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC5710.10902023079449208No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5610.10711094479108592No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5590.1067290875904047No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5350.1021468011822299No Hit
GTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTA5350.1021468011822299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14550.052.9507374
ATCAACG14650.052.6043663
CAACGCA14700.052.405425
TATCAAC15500.049.719612
AACGCAG15500.049.2361346
CGCAGAG16950.045.0198948
GTATCAA18450.044.137391
ACGCAGA19000.040.1624877
CTGTACG900.003754383231.995279
GTGCTAG1652.498071E-630.5730421
GTACATG15300.030.144851
AGACCGT1255.3650513E-428.7984926
GCAGAGT26750.028.2575049
ACATGGG15800.027.8066313
TACACCG1358.4132905E-426.665275
GTACACG3555.456968E-1226.3648175
TACACTA2508.361894E-825.9186445
TCTATAC1959.202995E-625.8546733
GTATAGG1701.07337706E-425.434721
TAGATTG1701.0803678E-425.4104355
Produced by FastQC (version 0.11.5)