FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506835
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA18960.3740862410843766No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCTCTCAGGC16160.31884143754870914No Hit
TGGTAACACCATTCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA15860.31292235145560193No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCTCTCAGG15720.31016011127881854No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15270.30128148213915773No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT15270.30128148213915773No Hit
GCTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT14760.29121903578087543No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC13720.27069953732477037No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA13380.2639912397525822No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12330.24327443842670693No Hit
ATGGTAACACCATTCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC11970.23617153511497824No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGACAGCTTATGATGTACACT11920.23518502076612705No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT11550.22788481458462811No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11230.22157112275198043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.21940079118450778No Hit
GTAATAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA10850.2140736137007113No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT10810.2132844022216303No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA10650.21012755630530647No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCATTCGACCCT10320.2036165616028885No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9770.19276490376552527No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG9630.1900026635887419No Hit
GTGTACATCATAAGCTGTCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA8110.16001262738366528No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7970.15725038720688195No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7930.15646117572780097No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7610.15014748389515326No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7590.14975287815561278No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA7580.14955557528584254No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7530.14856906093699132No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7460.14718794084859965No Hit
TCATAAGCTGTCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGAC7210.14225536910434364No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6970.13752010022985783No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6960.1373227973600876No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6930.13673088875077688No Hit
GATTATTACTGCCAGTCCTATGACAACAGCCTGAGAGGTTATGTCTTCGG6880.1357443744019257No Hit
GTTTATTACTGTGCGAGAGAGACGTACCAGAGTAACTTGGGGACCTGGTT6880.1357443744019257No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT6820.13456055718330423No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG6810.134363254313534No Hit
GTCTCTGGTGGCTCCTTCAGCAGTACTAACTGCTGGGGTTGGGTCCGCCA6770.133574042834453No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6440.1270630481320351No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT6380.12587923091341363No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6320.1246954136947922No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC6320.1246954136947922No Hit
GGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAAC6090.12015744769007665No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT6080.11996014482030641No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC6080.11996014482030641No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6020.11877632760168497No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC5910.11660599603421232No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5910.11660599603421232No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG5860.11561948168536111No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT5860.11561948168536111No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC5750.11344915011788848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.10891118411317292No Hit
TCCTATGACAACAGCCTGAGAGGTTATGTCTTCGGAACTGGGACCAAGGT5500.10851657837363245No Hit
TCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGT5460.10772736689455148No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT5450.10753006402478124No Hit
GTTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCC5370.1059516410666193No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG5330.10516242958753835No Hit
GTACTAACTGCTGGGGTTGGGTCCGCCAGCCCCCAGGGAAGGGACTGGAG5310.10476782384799786No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT5200.10259749228052521No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC5130.10121637219213354No Hit
GTCCTATGACAACAGCCTGAGAGGTTATGTCTTCGGAACTGGGACCAAGG5120.1010190693223633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.10042716071305258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14700.058.7726944
TATCAAC14650.058.4818342
ATCAACG14750.058.085353
GGTATCA5400.057.34191
GTATCAA15250.057.1364021
CAACGCA15300.056.4678765
AACGCAG15750.054.854516
CAGTGCG553.3300952E-452.3611264
ACGCAGA17050.050.6670537
CGCAGAG17150.050.3716248
GCAGAGT19050.045.3476839
CCCTATA750.001534343338.398162
GTGTAGA3400.038.1233441
TCTACAC6700.037.6101343
CTCTACA6550.035.1738852
TATTGGG3900.035.0752372
ATAGTAC1051.9298513E-434.2840733
CACAACC1708.303141E-833.880734
CAACCAT1501.1958145E-633.598396
GTGCTAG3950.032.815031
Produced by FastQC (version 0.11.5)