FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506835
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTAACACCATTCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA16840.3322580326930855No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT14960.29516509317628026No Hit
GTCCTGGGCCCAGTCTCTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC14790.2918109443901861No Hit
GCTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT14590.28786488699478135No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14520.28648376690638966No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCTCTCAGG13380.2639912397525822No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCTCTCAGGC13310.2626101196641905No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT13220.26083439383625834No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC13100.2584667593990154No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGACAGCTTATGATGTACACT12430.24524746712440934No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA12310.24287983268716645No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12150.23972298677084258No Hit
ATGGTAACACCATTCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC12000.23676344372428898No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA11860.2340012035475056No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT11560.22808211745439835No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT11260.22216303136129112No Hit
GTAATAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA11140.21979539692404826No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA10970.21644124813795418No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC10120.19967050420748367No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCATTCGACCCT9170.18092673157931083No Hit
CTGCACAGGGTCCTGGGCCCAGTCTCTGCTGACGCAGCCGCCCTCAGTGT7850.15488275276963903No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG7550.1489636666765318No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7480.14758254658814013No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7420.14639872936951867No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7400.1460041236299782No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7200.1420580662345734No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7180.1416634604950329No Hit
GTTTATTACTGTGCGAGAGAGACGTACCAGAGTAACTTGGGGACCTGGTT6990.13791470596939834No Hit
GATTATTACTGCCAGTCCTATGACAACAGCCTGAGAGGTTATGTCTTCGG6790.13396864857399352No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT6720.13258752848560182No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6690.1319956198762911No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC6630.13081180265766967No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG6620.13061449978789944No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC6600.13021989404835893No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6560.12943068256927795No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT6470.1276549567413458No Hit
GTCTCTGGTGGCTCCTTCAGCAGTACTAACTGCTGGGGTTGGGTCCGCCA6470.1276549567413458No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6390.12607653378318387No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA6240.12311699073663027No Hit
GCACAGGGTCCTGGGCCCAGTCTCTGCTGACGCAGCCGCCCTCAGTGTCT6180.12193317351800882No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6120.12074935629938738No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6080.11996014482030641No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG5830.11502757307605038No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC5760.1136464529876587No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT5730.11305454437834797No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC5720.11285724150857773No Hit
GTGTACATCATAAGCTGTCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA5570.10989769846202413No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT5560.10970039559225389No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT5540.10930578985271341No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG5400.10654354967593004No Hit
GGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAAC5320.10496512671776811No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT5320.10496512671776811No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC5240.10338670375960618No Hit
GTACTAACTGCTGGGGTTGGGTCCGCCAGCCCCCAGGGAAGGGACTGGAG5220.10299209802006572No Hit
TCCTATGACAACAGCCTGAGAGGTTATGTCTTCGGAACTGGGACCAAGGT5170.10200558367121451No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA5150.101610977931674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)