FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691381
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCACCTCTCAGTCTGTTCATTTGCAGATACA27180.393126221287539No Hit
GTGTATTACTGTGCGAGAGATTCCGGGGACAGCAGTGACTGGTACCTGTT19940.288408272717937No Hit
CTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACC18050.2610716811714525No Hit
CTCCTATAGTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC16340.23633857453415702No Hit
ATATTACACAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT15890.22982986226118451No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT15280.221006941180044No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG14780.213775038654519No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13170.1904883125223285No Hit
GTGTAATATTTATTACTTCCATCATACGATATAACTGCCACCCACTCCAG12420.179640458734041No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10940.158234027258487No Hit
CCTATAGTGGTGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA10860.157076922854403No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGTCCCCAAGGAG10800.15620909455134No Hit
CTCCTGGACACCTGCCCGATTCTCAGGCTCCCTCCTTGGGGACAAAGCTG10630.1537502476926615No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10490.1517253149855145No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10220.147820087621731No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG9460.136827595782933No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT9240.133645558671702No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG9130.1320545401160865No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACCACTA8960.129595693257408No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT8940.129306417156387No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8850.1280046747017925No Hit
TCATAAGGGTATCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8800.12728148444924No Hit
CCGTAATAGTTATAATCAAACAGGTACCAGTCACTGCTGTCCCCGGAATC8330.12048349607524649No Hit
GTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8000.11571044040839999No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7960.115131888206358No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7950.1149872501558475No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT7840.113396231600232No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGT7790.1126730413476795No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC7760.112239127196148No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7740.111949851095127No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT7670.1109373847415535No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGCTG7510.1086231759333855No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA7500.10847853788287501No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7410.10717679542828051No Hit
GTCCAGGAGTGTTTGTTGTTTGTATCATAAATTAGTGTCCTGGGGGCTTG7320.105875052973686No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAATTTC7110.10283765391296551No Hit
ATACACAGCCGTGTCCTCACCTCTCAGTCTGTTCATTTGCAGATACAGCG7110.10283765391296551No Hit
GTATTACTGTGCGAGAGATTCCGGGGACAGCAGTGACTGGTACCTGTTTG7030.1016805495088815No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGTACTGCT7010.1013912734078605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9600.050.2625273
TCAACGC9700.049.7443544
CAACGCA10200.046.5998465
TATCAAC10950.044.7234732
AACGCAG11000.043.2045146
CGCAGAG11250.042.244418
GTATCAA12050.041.2414781
GTGCTAG2501.8189894E-1234.5711171
GGTATCA7750.033.4559171
GCAGAGT14750.032.2179879
TACTAGG900.003747911432.0079772
GTACATG16850.031.630351
TATTGAG7100.030.4301175
TTATTGA7700.029.9295394
TACATGG16850.029.9184362
ACATGGG16750.028.807183
CAGCCGT13000.028.8009269
ACGCAGA16700.028.4580637
AATACAC13900.027.9781953
CCTATAC1856.1221035E-627.2500343
Produced by FastQC (version 0.11.5)