FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083795
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGTTATTCATTTGCAGATATA40260.3714724648111497No Hit
GTATATTACTGTGCGAAAGATGCCCCCTTAGAAGGATATTGTTCTGATAT26930.2484787252201754No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17080.1575943790107908No Hit
CCCTATATCTGCAAATGAATAACCTGAGAGCCGAGGACACGGCCGTATAT15390.14200102417892682No Hit
GATATAGGGTGTTCTTGGAATTGTCGCTGGAGATGATGAACCGGCCCTTC15330.14144741394820978No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGTTATTCATTTGCAGATATAGGG15140.13969431488427242No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14880.13729533721783177No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14010.12926798887243435No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13310.12280920284740195No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12640.11662722193772808No Hit
GCCGTATATTACTGTGCGAAAGATGCCCCCTTAGAAGGATATTGTTCTGA12500.1153354647327216No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11930.1100761675409095No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11600.10703131127196563No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11580.10684677452839329No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11490.1060163591823177No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11410.10527821220802827No Hit
CATCTATTCATGGTAATGGTAGGACATACTACGCAGACTCCGTGAAGGGC11350.10472460197731122No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC11330.10454006523373885No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11310.10435552849016651No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCATCATCTCCAGCGACAATT10860.10020345175978854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.059.2406431
ATCAACG13150.053.1947173
TCAACGC13150.053.1947174
CAACGCA14550.048.578715
AACGCAG15200.046.9171876
TATCAAC15700.045.0162242
GTACATG27000.037.141081
TACATGG26500.036.7392732
TAGGTCG800.002089261336.0622525
ACATGGG26650.035.9895943
GGTATCA6500.034.4074021
ATTGCGC1051.925329E-434.3021288
CGCAGAG20850.033.857938
CATGGGA15650.031.3340954
ATACGGC18850.030.9538046
TATACGG18700.030.8554045
CATGGGG15200.030.3639974
TACGGCC19150.030.092737
ATATACG19800.029.8655974
GCAGAGT24300.028.4580639
Produced by FastQC (version 0.11.5)