FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216120
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.12062954313719042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.065.9591451
TCAACGC23300.059.7025954
ATCAACG24250.057.6609543
CAACGCA24700.056.6104475
AACGCAG25900.053.9831246
TATCAAC27800.050.8163072
ACGCAGA29150.047.9486357
CGCAGAG31400.044.4817168
GGTATCA13500.044.3226281
GCAGAGT38550.036.605069
TACCGTC1453.6437472E-529.8123597
GTACATG32000.028.3858411
TACATGG31300.027.1724932
GTACCGT1358.369334E-426.6926886
GTATTAG6850.026.3105931
TAGACGG1658.762548E-526.2095244
ACATGGG31350.026.2095223
GTATACG1400.001033126525.7467941
CAGAGTA35500.024.133939
ATTGCCG7500.022.0788298
Produced by FastQC (version 0.11.5)