FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216120
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13500.051.2800184
ATCAACG14100.048.0750163
GTATCAA14500.047.269531
CAACGCA15650.043.313595
AACGCAG16450.041.5906526
ACGCAGA18100.037.4013527
TATCAAC18400.036.8400962
CGCAGAG21000.032.2364048
GGTATCA11100.031.1992341
TTTCGCG1851.7426464E-731.1439869
GTACATG30950.030.0714741
TACATGG31100.028.9841332
ACATGGG30200.028.1764183
GCAGAGT25500.026.8311549
AATAGCG1952.7117337E-422.1884695
ACACCGT2404.5942965E-521.0051046
ATATAAG6650.020.613781
TCTACGC2206.1213446E-419.6670532
ATAATAC8550.019.3986933
ATTGCCG5950.019.366058
Produced by FastQC (version 0.11.5)