FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034843
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCGTCAGATCTCAGACTCTTCAACTCCAGGTAGA39170.3785115230039726No Hit
ATGTATTACTGTGCGAGGGGTTGTGGTGTTAGCAGCTGCGCTGGATTTGA36760.3552229661890741No Hit
GTATTACTGTGCGAGGGGTTGTGGTGTTAGCAGCTGCGCTGGATTTGATA30100.29086537764665754No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25840.24969971290330997No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24580.23752395290879874No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24320.2350114944972329No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC23810.23008321068993076No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23310.22525155989845802No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22630.21868051482205514No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20940.20234953514687734No Hit
CAGTAATACATGGCCGTGTCGTCAGATCTCAGACTCTTCAACTCCAGGTA19030.18389262912345158No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17950.17345626341387052No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17720.17123370404979305No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG15990.15451619231129748No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15520.1499744405673131No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15500.1497811745356542No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT15430.14910474342484803No Hit
GCCATGTATTACTGTGCGAGGGGTTGTGGTGTTAGCAGCTGCGCTGGATT14760.14263033136427458No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14650.1415673681901506No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14330.13847511168360804No Hit
GTCTGGGGCTGAGGTGATGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA14130.13654245136701895No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13190.12745894787905024No Hit
ATACATGGCCGTGTCGTCAGATCTCAGACTCTTCAACTCCAGGTAGATTG13180.12736231486322078No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12890.12455995740416662No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG12860.12427005835667826No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12680.12253066407174808No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12390.11972830661269392No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12320.11905187550188773No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12310.11895524248605827No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12090.11682931613781027No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11700.11306062852046155No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11640.11248083042548482No Hit
CTCCCAGGTGCAACTGCTGCAGTCTGGGGCTGAGGTGATGAAGCCTGGGG11530.11141786725136084No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11520.11132123423553138No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11210.10832561074481831No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT11190.10813234471315938No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11190.10813234471315938No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10950.10581315233325247No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10920.10552325328576412No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10880.1051367212224463No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10790.1042670240799812No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC10680.10320406090585721No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10650.10291416185836885No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT10620.1026242628108805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.078.7978741
GTATCAA14450.066.3898851
TCAACGC15600.060.9536864
CAACGCA15800.060.17635
AACGCAG16800.056.9762846
ATCAACG17600.054.0271263
TATCAAC18050.053.4783822
CGCAGAG20450.046.1030588
CAGCGCT6850.044.127629
GCAGAGT23800.039.613769
ACGCAGA24900.038.441837
GCAGCGC7800.037.830418
GGCAGCG8550.034.5119487
AGCGTTA850.002831015533.89615
GTACATG22000.033.7701261
ACATGGG22500.031.6959193
TAGCGTT1000.006268025428.814474
TACACGG13000.028.7880156
GTATGTA14050.028.2360841
ACGTGCT21000.027.785383
Produced by FastQC (version 0.11.5)