FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034843
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCGTCAGATCTCAGACTCTTCAACTCCAGGTAGA37840.3656593318986552No Hit
ATGTATTACTGTGCGAGGGGTTGTGGTGTTAGCAGCTGCGCTGGATTTGA33340.32217447477540073No Hit
GTATTACTGTGCGAGGGGTTGTGGTGTTAGCAGCTGCGCTGGATTTGATA25880.2500862449666278No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25080.24235560370027145No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22350.2159747903788304No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22270.21520172625219477No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21310.20592495673256717No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20510.1981943154662108No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19740.19075357324734285No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18920.1828296659493276No Hit
CAGTAATACATGGCCGTGTCGTCAGATCTCAGACTCTTCAACTCCAGGTA17880.17277983230306432No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17310.16727175040078543No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15300.1478485142190651No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14860.1435966615225691No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14480.13992460692104988No Hit
GCCATGTATTACTGTGCGAGGGGTTGTGGTGTTAGCAGCTGCGCTGGATT14240.13760541454114295No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14190.1371222494619957No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13810.13345019486047643No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13610.13151753454388732No Hit
GTCTGGGGCTGAGGTGATGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA13360.12910170914815097No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT13110.12668588375241463No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13050.1261060856574379No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG12820.12388352629336044No Hit
ATACATGGCCGTGTCGTCAGATCTCAGACTCTTCAACTCCAGGTAGATTG12760.12330372819838371No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12750.12320709518255427No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12580.12156433391345353No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12460.12040473772350008No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG12310.11895524248605827No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11320.1093885739189423No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11260.10880877582396557No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11060.1068761155073765No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10890.10523335423827576No Hit
CTCCCAGGTGCAACTGCTGCAGTCTGGGGCTGAGGTGATGAAGCCTGGGG10830.10465355614329903No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10520.10165793265258594No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10420.1006916024942914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11050.061.235791
GGTATCA5650.052.2366261
CAACGCA12950.052.1957665
TCAACGC13150.051.3919684
TAGACGA450.00996415347.9293864
ATCAACG14200.047.0813
TATCAAC14250.046.9158062
AACGCAG14900.046.3411946
CGCAGAG16450.042.8491678
CAGCGCT6100.038.910469
GTACACG12700.033.9722825
GCAGAGT20350.033.5769659
GTATGTA14650.032.429931
ATACCGT1352.2620425E-531.9668456
TACACGG13350.031.7872526
ACGCAGA22050.031.640657
GTACATG25500.031.6167761
GCAGCGC8100.031.0788738
ACGGGGT13750.030.8625349
GGCAGCG7800.030.4299747
Produced by FastQC (version 0.11.5)