FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962645
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC38160.19443149423354705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29160.14857500974450297No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCAAATACGGGATGACCATTA28500.14521220088197304No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG28380.1446007810887858No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25080.12778673677613628No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCAAATACGGG22320.11372408153282941No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20830.10613228576742101No Hit
GCCTCTGTCTCCTCCTATGTGCTCACTCAGCCACCCTCAGTGTCAGTGGC20150.10266757360602656No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGGCCCTGG19680.10027284608270981No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39450.070.847481
GGTATCA17100.066.558061
TCAACGC43800.062.632894
ATCAACG44750.061.142353
CAACGCA45500.060.1345025
AACGCAG48200.056.912466
TATCAAC50200.054.3609472
ACGCAGA59100.046.4147347
CGCAGAG59300.046.258198
GCAGAGT68050.040.4160279
GTACATG57250.030.7011071
ACATGGG57900.028.8510063
TACATGG62300.027.853552
GCGTTAA1358.39557E-426.6793921
CAGAGTA66250.025.5387949
ACCGTTC21650.025.2740068
CATGGGA26700.024.5404014
TGGACCG24550.023.170065
TATACCG1600.002264599522.5009865
GACCGTT24350.022.1758697
Produced by FastQC (version 0.11.5)