FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962645
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC32730.1667647485918238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27300.1390980029501005No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCAAATACGGGATGACCATTA25780.13135335223639527No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG24580.12523915430452273No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22150.1128579034924808No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCAAATACGGG21500.10954604627938318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38800.070.1452261
TCAACGC43400.061.0530244
ATCAACG45000.059.0422553
GGTATCA18600.055.7426451
CAACGCA47500.055.63165
TATCAAC48900.054.6278532
AACGCAG49400.053.4919246
CGCAGAG54950.048.2202228
ACGCAGA55350.047.611587
GCAGAGT65800.040.3722729
GTACATG56000.030.6003911
ACATGGG57050.028.649653
TACATGG61750.027.2852542
CAGAGTA64150.025.4748659
GTACACG6550.025.283415
TACACGG6900.024.0009176
TATTGAG19950.022.737715
CATGGGA26350.022.1336734
CTAGTAC4307.8216544E-1121.7682743
CCTAGTA5800.021.1042562
Produced by FastQC (version 0.11.5)