FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631477
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC108030.41052990392847816No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTGGATGATCACTA95920.36451012112209225No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG86680.3293967608305146No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTGG85580.32521659889104104No Hit
TTATGATACCGACCGACCCTCAGGGATCCCTGACCGATTCTCTGGCTCCA75970.28869718412891315No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC75770.2879371546853725No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65460.2487576368708524No Hit
GTATCATAATAGATGACCAGAACAGGGGCCTGGCCTGGCTTCTGCTGATA61300.23294902444520701No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAGCGACG60310.22918687869968082No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA59460.22595675356463307No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA59370.2256147403150398No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT57320.21782443851874825No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA56750.2156583546046574No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA54990.2089700955014997No Hit
ACCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAACTCTGGGAACACGG52340.1988997053745862No Hit
ATCTATTACTGTGCGAGGCCTTCCGATTCCAGTGATCGGGGAGGTGCTTT51840.1969996317657346No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50320.19122340799482573No Hit
CCCCTGTTCTGGTCATCTATTATGATACCGACCGACCCTCAGGGATCCCT49170.18685323869446704No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT48620.18476315772473026No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTGGATGATCACTAC48510.1843451415307829No Hit
GTAATAGATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAAGTAGG48220.18324309883764897No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC47590.18084900609049595No Hit
GTGGATGATCACTACTACTTTCCCACACCTGACAGAAATAGTCGGCCTCA44880.17055060713052023No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA44670.16975257621480255No Hit
GTTCTGGTCATCTATTATGATACCGACCGACCCTCAGGGATCCCTGACCG43910.16686446432934812No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42880.16295031269511379No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA42830.16276030533422867No Hit
GAACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATGCACACTTTTAGTT41970.15949217872700389No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC41440.1574781007016212No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT40880.15535001825970737No Hit
GGTATCAGCAGAAGCCAGGCCAGGCCCCTGTTCTGGTCATCTATTATGAT40870.15531201678753034No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG40610.15432397851092752No Hit
CTACTACTTTCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTTC40140.152537909318607No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC39750.15105585190370274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT39220.14904177387832004No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT38760.14729370615817655No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37810.14368356630135853No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36820.13992142055583234No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36290.13790734253044962No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCA36100.13718531455908603No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA36050.13699530719820086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG35710.13570325714418177No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG34780.13216912023171778No Hit
GTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAGCGACGGCCAG34640.13163709962123932No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC31860.12107269035602439No Hit
GTAGTAGTGATCATCCACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA31120.11826058141492402No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA30160.11461244008592893No Hit
ATCATCCACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC30040.11415642241980455No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29670.11275036794925436No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29150.11077429139604868No Hit
TCTATTATGATACCGACCGACCCTCAGGGATCCCTGACCGATTCTCTGGC29140.11073628992387165No Hit
GGATTACCTGTGGGGGAAACAACATTGGAACTAAAAGTGTGCATTGGTAT29090.11054628256298649No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG29070.11047027961863243No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT28350.10773417362188611No Hit
ATTATGATACCGACCGACCCTCAGGGATCCCTGACCGATTCTCTGGCTCC28300.10754416626100095No Hit
CACTACTACTTTCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCT27630.1049980676251398No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG27260.1035920131545896No Hit
CCCTCAGTGTCAGTGGCCCCAGGAGCGACGGCCAGGATTACCTGTGGGGG27130.1030979940162882No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26980.10252797193363271No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT26970.10248997046145567No Hit
AAGTAGTAGTGATCATCCACTGTTCGGCGGAGGGACCAAGCTGACCGTCC26920.1022999631005705No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG26690.10142592924049879No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA26560.10093191010219736No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46150.067.710871
TCAACGC48300.063.6509064
ATCAACG49800.061.878283
TATCAAC53850.057.491892
CAACGCA54200.056.7221155
AACGCAG56550.054.4922836
ACGCAGA60700.050.5294727
CGCAGAG63400.048.604718
GTACATG71850.042.1886561
TACATGG73150.041.338892
GCAGAGT76050.040.4229249
ACATGGG85000.035.6604653
TATTGAG47300.034.705445
TTATTGA49300.033.4435544
TGGGAGT32100.032.747016
ATGGGAG38300.031.9575885
GGTTTAT36850.030.2854881
ATTGAGT36250.029.5938936
GAATACG5200.029.0746889
GTAATAG26950.028.8539541
Produced by FastQC (version 0.11.5)