FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892029
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG60850.6821527102818407No Hit
GATTACTACTGTGCAGCATGGGATGGCAGCCTGAGTGGTCTTTGGGTGTT41080.4605231444269189No Hit
GTAATATACGGCCGTGTCCTCGGATTCCAGGCTGCTCAGCTCCATGTAGG39210.43955970041332737No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36750.4119821216574797No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACCACTC23270.2608659583937294No Hit
GTTATGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATAGG22800.2555970713956609No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT22530.2525702639712386No Hit
GTATATTACTGTGCGGCCGACCCTTCCTTTGGAGTGGTTAGTCGACACTA22470.2518976400991447No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21730.24360194567665402No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC21650.24270511384719556No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21510.24113565814564325No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA21500.24102355416696095No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20720.23227944382974097No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20430.229028428447954No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20180.22622582898089638No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19320.21658488681421795No Hit
CCCAAAGACCACTCAGGCTGCCATCCCATGCTGCACAGTAGTAATCAGCC18350.20571080088203408No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG18280.20492607303125798No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18100.2029082014149764No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA17830.19988139399055413No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17430.19539723484326182No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16850.188895204079688No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16840.18878310010100569No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16370.18351421310293722No Hit
ATATACGGCCGTGTCCTCGGATTCCAGGCTGCTCAGCTCCATGTAGGCTG16310.1828415892308434No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16300.18272948525216107No Hit
CTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCG16060.1800389897637857No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15900.1782453261048688No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15590.17477010276571725No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA15230.1707343595331542No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14920.16725913619400265No Hit
CTGTTATGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATA14840.1663623043645442No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14810.16602599242849728No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14560.1632233929614396No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14540.16299918500407498No Hit
GCCTACATGGAGCTGAGCAGCCTGGAATCCGAGGACACGGCCGTATATTA14430.1617660412385696No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14430.1617660412385696No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA14410.16154183328120497No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA14340.16075710543042884No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14130.15840292187810037No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14110.15817871392073576No Hit
ATCCCATGCTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCAC14020.157169778112595No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13540.15178878713584423No Hit
GTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC13450.15077985132770347No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT13440.15066774734902116No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13270.14876197971142194No Hit
TATGTATACTGGTACCAGCATCTCCCAGGAACGGCCCCCAGACTCCTCAT13260.14864987573273963No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12960.1452867563722704No Hit
GTATACATAATTATCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGG12950.14517465239358812No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT12910.14472623647885888No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACCACTCA12900.14461413250017657No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12480.13990576539551966No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12270.1375515818431912No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12200.13676685399241503No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12160.13631843807768582No Hit
CCCAGGAACGGCCCCCAGACTCCTCATCTCTAGGAATAATCAGCGGCCCT12010.1346368783974512No Hit
TAGTAGTAGTGTCGACTAACCACTCCAAAGGAAGGGTCGGCCGCACAGTA11900.13340373463194583No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11690.13104955107961735No Hit
GCCGTATATTACTGTGCGGCCGACCCTTCCTTTGGAGTGGTTAGTCGACA11310.12678959988968969No Hit
GGATAGGATGGATCGTCGTTGGCAGTGGTAACACAAACTACGCACAGAAG11180.1253322481668197No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11060.123987000422632No Hit
TAATTATGTATACTGGTACCAGCATCTCCCAGGAACGGCCCCCAGACTCC11000.12331437655053816No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10770.12073598504084508No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10470.11737286568037587No Hit
GCATGGGATGGCAGCCTGAGTGGTCTTTGGGTGTTCGGCGGAGGGACCAA10330.11580340997882357No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA10300.11546709804277663No Hit
ATATTACTGTGCGGCCGACCCTTCCTTTGGAGTGGTTAGTCGACACTACT10270.11513078610672972No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG10120.1134492264264951No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC10100.11322501846913048No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10070.11288870653308357No Hit
TTACTACTGTGCAGCATGGGATGGCAGCCTGAGTGGTCTTTGGGTGTTCG10030.11244029061835434No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG10030.11244029061835434No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9910.11109504287416663No Hit
CCGTAGTAGTAGTAGTGTCGACTAACCACTCCAAAGGAAGGGTCGGCCGC9860.1105345229807551No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG9850.11042241900207281No Hit
CAGTAATATACGGCCGTGTCCTCGGATTCCAGGCTGCTCAGCTCCATGTA9780.10963769115129665No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA9690.10862875534315589No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9500.10649877974819204No Hit
CATGTAGGCTGTGCTTGTGGACATGTCCCTAGTAATGGTGACTCTTTCCT9500.10649877974819204No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9460.10605036383346282No Hit
GTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCTCAGCCTCC9380.10515353200400435No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9340.10470511608927512No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCT9310.1043688041532282No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG9260.10380828425981667No Hit
TTACTAGGGACATGTCCACAAGCACAGCCTACATGGAGCTGAGCAGCCTG9230.10347197232376976No Hit
CCATGTAGGCTGTGCTTGTGGACATGTCCCTAGTAATGGTGACTCTTTCC9150.10257514049431128No Hit
ATTATGTATACTGGTACCAGCATCTCCCAGGAACGGCCCCCAGACTCCTC9140.10246303651562899No Hit
GCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAG9130.10235093253694667No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9090.10190251662221744No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9050.1014541007074882No Hit
CAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA9000.10089358081407666No Hit
ACATAATTATCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGAC8980.10066937285671206No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8970.10055726887802975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)