FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892029
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTACTGTGCAGCATGGGATGGCAGCCTGAGTGGTCTTTGGGTGTT40760.45693581710908504No Hit
GTAATATACGGCCGTGTCCTCGGATTCCAGGCTGCTCAGCTCCATGTAGG39450.4422501959017028No Hit
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG36700.41142160176406817No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35470.3976328123861444No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC25650.2875467053201185No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23860.2674800931359855No Hit
GTATATTACTGTGCGGCCGACCCTTCCTTTGGAGTGGTTAGTCGACACTA23830.2671437811999386No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA23030.258175462905354No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACCACTC22620.25357919977937937No Hit
GTTATGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATAGG22270.2496555605254986No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21800.24438667352743018No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21580.2419203859964194No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20920.2345215234033871No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20650.23149471597896482No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20210.2265621409169433No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19620.21994800617468713No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18800.21075547992273794No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17760.19909666613977797No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA17300.19393988312039184No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17210.19293094731225105No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16940.18990413988782875No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG16720.187437852356818No Hit
CTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCG16140.18093582159324417No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15970.17903005395564492No Hit
ATATACGGCCGTGTCCTCGGATTCCAGGCTGCTCAGCTCCATGTAGGCTG15940.178693742019598No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15810.17723639029672802No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15570.17454589480835264No Hit
TATGTATACTGGTACCAGCATCTCCCAGGAACGGCCCCCAGACTCCTCAT15330.17185539931997726No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA15080.16905279985291957No Hit
GTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC14940.16748334415136729No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA14800.16591388844981497No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14750.16535336855640342No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14650.16423232876958035No Hit
CCCAAAGACCACTCAGGCTGCCATCCCATGCTGCACAGTAGTAATCAGCC14590.1635597048974865No Hit
GTATACATAATTATCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGG14440.16187814521725188No Hit
GCCTACATGGAGCTGAGCAGCCTGGAATCCGAGGACACGGCCGTATATTA14320.1605328974730642No Hit
CCCAGGAACGGCCCCCAGACTCCTCATCTCTAGGAATAATCAGCGGCCCT14310.1604207934943819No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14210.15929975370755883No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA14180.15896344177151192No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14020.157169778112595No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14000.1569455701552304No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT13550.15190089111452654No Hit
CTGTTATGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATA13550.15190089111452654No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13480.1511161632637504No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13410.15033143541297425No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13400.15021933143429192No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13380.14999512347692734No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13350.1496588115408804No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13320.1493224996048335No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13280.14887408369010424No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT13170.14764093992459887No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACCACTCA12980.14551096432963503No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12520.14035418131024888No Hit
TAATTATGTATACTGGTACCAGCATCTCCCAGGAACGGCCCCCAGACTCC12390.13889682958737887No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12330.13822420571528504No Hit
TAGTAGTAGTGTCGACTAACCACTCCAAAGGAAGGGTCGGCCGCACAGTA12110.13575791818427427No Hit
GCCGTATATTACTGTGCGGCCGACCCTTCCTTTGGAGTGGTTAGTCGACA12100.13564581420559196No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11730.1314979669943466No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11470.1285832635486066No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11260.12622907999627814No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11150.12499593623077276No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10860.12174492084898585No Hit
ATTATGTATACTGGTACCAGCATCTCCCAGGAACGGCCCCCAGACTCCTC10570.11849390546719894No Hit
GGATAGGATGGATCGTCGTTGGCAGTGGTAACACAAACTACGCACAGAAG10420.11681234578696434No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10370.11625182589355279No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC10240.11479447417068278No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT10220.11457026621331817No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA10210.11445816223463587No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10160.11389764234122433No Hit
ATATTACTGTGCGGCCGACCCTTCCTTTGGAGTGGTTAGTCGACACTACT10000.1121039786823074No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG9980.1118797707249428No Hit
CCGTAGTAGTAGTAGTGTCGACTAACCACTCCAAAGGAAGGGTCGGCCGC9860.1105345229807551No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9850.11042241900207281No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9820.11008610706602587No Hit
GTATACTGGTACCAGCATCTCCCAGGAACGGCCCCCAGACTCCTCATCTC9790.10974979512997896No Hit
GCATGGGATGGCAGCCTGAGTGGTCTTTGGGTGTTCGGCGGAGGGACCAA9770.10952558717261433No Hit
CATGTAGGCTGTGCTTGTGGACATGTCCCTAGTAATGGTGACTCTTTCCT9700.10874085932183819No Hit
TTACTACTGTGCAGCATGGGATGGCAGCCTGAGTGGTCTTTGGGTGTTCG9650.10818033942842666No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9620.10784402749237973No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9570.10728350759896818No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG9420.10560194791873359No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG9400.10537773996136897No Hit
CCATGTAGGCTGTGCTTGTGGACATGTCCCTAGTAATGGTGACTCTTTCC9390.10526563598268665No Hit
GATTATTCCTAGAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGATGCTGG9220.10335986834508744No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9220.10335986834508744No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA9190.1030235564090405No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG9100.10201462060089973No Hit
CAGTAATATACGGCCGTGTCCTCGGATTCCAGGCTGCTCAGCTCCATGTA9090.10190251662221744No Hit
TTACTAGGGACATGTCCACAAGCACAGCCTACATGGAGCTGAGCAGCCTG9070.10167830866485282No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9060.10156620468617052No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC8950.10033306092066513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.059.7156871
TCAACGC10900.059.469374
ATCAACG11100.059.0467153
TATCAAC11550.056.132062
CAACGCA11800.054.3171045
GGTATCA4550.052.254791
AACGCAG12600.051.4284136
CGCAGAG13100.049.4599468
GCAGAGT15200.042.1530349
TATTGAG15050.039.7164275
TTATTGA16500.037.1032834
ACATGGG20200.036.012013
TTTATTG18550.033.7794573
TCTACAC5200.033.2418563
ACGCAGA19750.032.8100247
GGTTTAT11650.032.777331
GTGCTAG2206.657501E-1032.7492141
GTACATG22300.032.631731
ATTGAGG6400.032.6248976
ATTGAGT11600.032.275766
Produced by FastQC (version 0.11.5)