FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357646
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC43130.3176822234956682No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGGTACTA30880.22745251707735303No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC30040.22126533720866853No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCAGTGGATCCAGTAGTGACGTT28580.21051142934166933No Hit
GTATATGAGCAGCAGTAATAATAAGCCTCGTCCTCAGCCTGGAGTCCAGA28310.20852269295530645No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTTATTATTACTGCTGCTCATAT25330.1865729358021163No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT25030.18436322870615757No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT24500.18045941283663047No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA24140.17780776432147996No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA23730.17478783129033637No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC23100.170147446388823No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23020.169558191163234No Hit
GCTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGTCCCCAA22670.16698019955128215No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22400.16499146316491928No Hit
GTACCAACACCACCCAGGCAAAGTCCCCAAAGTCATGATTTATGATGTCA21500.15836234187704307No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21170.15593166407148845No Hit
GCTCATATACAGGCACTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAG20530.15121762226677646No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20490.15092299465398196No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA20340.1498181411060026No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20340.1498181411060026No Hit
GTACTAGTGCCTGTATATGAGCAGCAGTAATAATAAGCCTCGTCCTCAGC19980.1471664925908521No Hit
CTCCTGCAGTGGATCCAGTAGTGACGTTGGTGCTTATAACTATGTCTCCT19620.14451484407570162No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA19500.1436309612373181No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19480.14348364743092087No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19420.1430417060117291No Hit
GTAATATACGGCCGTGTCCGCGACGGTCACAGAGCTCAGCCTCAGGGAGA19400.14289439220533187No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT19250.1417895386573525No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT18900.13921154704540065No Hit
TATTATTACTGCTGCTCATATACAGGCACTAGTACCCTCTGGGTGTTCGG18620.13714915375583916No Hit
TAATAAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGCGGCCGT18600.1370018399494419No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18510.1363389278206543No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT18430.1357496725950653No Hit
TCTCCAGCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGC17880.13169854291914093No Hit
GTATATTACTGTGCGCGACGATACTGTAGTAGTACCAGTTGTTATTACTG17810.13118294459675056No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17640.12993077724237392No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGGTACTAG16400.12079732124574447No Hit
CACTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG16300.12006075221375823No Hit
CTCATATACAGGCACTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAGC16150.11895589866577885No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16130.1188085848593816No Hit
CTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT16010.11792470202099811No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15870.11689350537621736No Hit
GTAATAATAAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGCGG15770.1161569363442311No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15250.11232677737790264No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15110.11129558073312189No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCCAATCGCTTCTCTGGCTACAAGTC15110.11129558073312189No Hit
GTCCAGAGATGGTCAGGGCGGCCGTGTCGCCAGACTTGTAGCCAGAGAAG14960.11019072718514251No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14940.11004341337874526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.10930684434675904No Hit
GGGTACTAGTGCCTGTATATGAGCAGCAGTAATAATAAGCCTCGTCCTCA14560.10724445105719754No Hit
GGCTTATTATTACTGCTGCTCATATACAGGCACTAGTACCCTCTGGGTGT14390.10599228370282092No Hit
GTCGATCACCATCTCCTGCAGTGGATCCAGTAGTGACGTTGGTGCTTATA14370.10584496989642367No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14000.10311966447807455No Hit
ATATTACTGTGCGCGACGATACTGTAGTAGTACCAGTTGTTATTACTGGT13730.10113092809171167No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13640.10046801596292405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)