FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357646
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC39440.29050282621537576No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC29400.21655129540395657No Hit
GTATATGAGCAGCAGTAATAATAAGCCTCGTCCTCAGCCTGGAGTCCAGA28210.20778612392332022No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCAGTGGATCCAGTAGTGACGTT27100.19961020766827287No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGGTACTA26870.19791609889470452No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTTATTATTACTGCTGCTCATAT23870.1758190279351171No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA22970.1691899066472409No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22830.16815871000246013No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22350.16462317864892614No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT21190.1560789778778857No Hit
GCTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGTCCCCAA21090.15534240884589945No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC20490.15092299465398196No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20000.14731380639724936No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19950.14694552188125623No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19750.14547238381728372No Hit
CTCCTGCAGTGGATCCAGTAGTGACGTTGGTGCTTATAACTATGTCTCCT19300.14215782317334563No Hit
GCTCATATACAGGCACTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAG19040.1402427436901814No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18770.1382540073038185No Hit
GTACCAACACCACCCAGGCAAAGTCCCCAAAGTCATGATTTATGATGTCA18400.1355287018854694No Hit
GTACTAGTGCCTGTATATGAGCAGCAGTAATAATAAGCCTCGTCCTCAGC18100.13331899478951068No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17880.13169854291914093No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17150.12632158898564133No Hit
TATTATTACTGCTGCTCATATACAGGCACTAGTACCCTCTGGGTGTTCGG17060.1256586768568537No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16980.1250694216312647No Hit
GTAATATACGGCCGTGTCCGCGACGGTCACAGAGCTCAGCCTCAGGGAGA16980.1250694216312647No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16750.12337531285769633No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16690.1229333714385046No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16620.12241777311611422No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT16440.12109194885853898No Hit
GTATATTACTGTGCGCGACGATACTGTAGTAGTACCAGTTGTTATTACTG16360.12050269363294996No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16170.1191032124721761No Hit
TCTCCAGCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGC15910.11718813298901186No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT15900.11711447608581324No Hit
GTAATAATAAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGCGG15300.11269506189389576No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14870.1095278150563549No Hit
TAATAAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGCGGCCGT14840.10930684434675904No Hit
CTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT14680.10812833389558102No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14590.1074654217667934No Hit
CTCATATACAGGCACTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAGC14500.10680250963800578No Hit
CACTAGTACCCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG14440.10636056821881404No Hit
GTCCAGAGATGGTCAGGGCGGCCGTGTCGCCAGACTTGTAGCCAGAGAAG13820.1017938402204993No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13800.10164652641410205No Hit
GGCTTATTATTACTGCTGCTCATATACAGGCACTAGTACCCTCTGGGTGT13760.10135189880130756No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGGTACTAG13750.10127824189810893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17450.058.9563331
TCAACGC18250.054.3467374
ATCAACG18450.053.755633
GGTATCA8150.052.0815931
CAACGCA19200.051.665335
AACGCAG19900.050.2239886
TATCAAC20350.048.736682
CGCAGAG23450.041.3958178
ACGCAGA25450.038.7063527
GCAGAGT27000.036.219359
TACATGG35300.033.5931322
GTACATG36100.033.48051
ACATGGG36400.031.5907463
TATTGAG20600.030.7069595
GAATACG3251.8189894E-1228.7624249
TAGACCG1000.006334569328.7528785
GGTTTAT16850.027.7525751
CATGGGG19800.027.5870784
ATTGAGT16100.026.79636
CTTACCG1358.482068E-426.6318749
Produced by FastQC (version 0.11.5)