FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421321
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31400.12968127728624168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43900.073.1513751
ATCAACG49100.064.522973
TCAACGC50350.062.6351134
GGTATCA22150.054.286891
TATCAAC60350.052.7336962
CAACGCA60250.052.2236945
ACGCAGA62650.049.9932567
CGCAGAG63900.049.015298
AACGCAG64800.048.445646
GCAGAGT80750.039.051619
GTACATG62550.026.5911331
TACATGG63350.025.118222
ACATGGG67450.023.3778933
CAGAGTA80450.021.2989529
GTATAGG11150.020.0188451
GCCCATA14550.019.2998451
GTATATA26500.019.291441
ACCGTAC2257.19789E-419.2004748
CCTATAC11250.019.2004743
GTACCGT2359.641612E-418.3834326
Produced by FastQC (version 0.11.5)