FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771508
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16330.21166339169522547No Hit
TCTATGATGCGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC12710.16474229690424466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12210.1582614827065954No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCT12070.1564468547312536No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC11340.14698486600268565No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10900.14128174950875427No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9950.12896820253322064No Hit
CCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTGGCAAC9560.12391316745905422No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9380.12158007434790047No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9210.11937659752069972No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8820.11432156244653328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.11406232987862731No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8720.11302539960700343No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8640.11198846933537954No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT8520.11043307392794371No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8390.10874806223655491No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8350.10822959710074297No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8230.10667420169330713No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCGGAAACGCCTGG8180.1060261202735422No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACACCCTAGAGCCTGAAGATT8180.1060261202735422No Hit
ACATAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCGTCCAACA7840.1016191666191407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7780.10084146891542278No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7750.10045262006356383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)