FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771508
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16300.2112745428433665No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12420.1609834246696081No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12170.15774301757078343No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCT11800.152947215064523No Hit
TCTATGATGCGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC11570.14996604053360432No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC10640.13791172612597666No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10010.12974590023693858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9640.1249500977306781No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9140.11846928353302882No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9090.1178212021132639No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8790.11393271359467433No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8560.11095153906375566No Hit
CCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTGGCAAC8530.1105626902118967No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT8530.1105626902118967No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCGGAAACGCCTGG8490.11004422507608475No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8410.10900729480446088No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7960.10317456202657653No Hit
ACATAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCGTCCAACA7880.10213763175495265No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7850.10174878290309369No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7840.1016191666191407No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7730.10019338749565786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11600.039.104914
TTACACG1303.901896E-738.770684
ATCAACG11900.038.124023
CAACGCA12300.036.879435
AACGCAG12700.036.2824676
TATCAAC12800.036.008352
GTATCAA13950.035.109471
CGCAGAG13200.034.905878
CTCTACG850.002832981533.8902132
TACACGG5350.032.298186
TACACCG1153.291647E-431.3055155
GTACATG20500.030.2158031
GTACACG5500.030.1102165
TACATGG20400.029.6539342
ACATGGG20800.029.0817813
GCAGAGT16600.028.1901689
ACGCAGA17100.026.946637
ACGGGGT6300.026.2832899
GTATGTA7800.025.8555011
CATGGGG11750.025.7371334
Produced by FastQC (version 0.11.5)