FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185176
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCATTATAGTGGGGGCACCAACTACAACCCGTCCCTCCAGAGTCGAGT42510.35868090477701203No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32170.2714364786327094No Hit
CCATTATAGTGGGGGCACCAACTACAACCCGTCCCTCCAGAGTCGAGTCA24570.20731098166010786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22450.1894233430309085No Hit
ATAATGGATAGTCCCAATCCACTCCGGCCCCTTCCCTGGGGGCTGGCGGA18200.15356369011859844No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17210.14521050038137795No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16850.1421729768405705No Hit
ACAGTATCCCGCTCACTTTCGGCGGTGGGACCAAACTAGATATCAGACGA16770.14149797160927996No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA16640.14040108810843283No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG16470.13896670199194044No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16130.13609792975895563No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15960.13466354364246322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15440.1302760096390747No Hit
GTCTACAGAGTGACAGTATCCCGCTCACTTTCGGCGGTGGGACCAAACTA15400.12993850702342943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.12749161306000123No Hit
GGATTGGGACTATCCATTATAGTGGGGGCACCAACTACAACCCGTCCCTC14820.125044719096573No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14790.12479159213483904No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14510.12242907382532214No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA14280.12048843378536184No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC14120.11913842332278075No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13860.11694465632108648No Hit
ATCCAGTGACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCA13740.11593214847415068No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13720.11576339716632804No Hit
GTTTAAAGCTGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCAGCCAGTT13700.1155946458585054No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG13590.1146665136654809No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13550.11432901104983563No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13480.1137383814724564No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13120.11070085793164898No Hit
ACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCAGCAGACACGTCG12970.10943522312297921No Hit
GGATACTGTCACTCTGTAGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC12790.10791646135257547No Hit
GATTGGGACTATCCATTATAGTGGGGGCACCAACTACAACCCGTCCCTCC12700.10715708046737363No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12610.10639769958217175No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12450.10504768911959068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12340.10411955692656617No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12290.1036976786570096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25150.065.118511
ATCAACG28500.057.882393
TCAACGC28700.057.730034
TATCAAC30950.053.9986842
ACGCAGA34800.047.5644847
CGCAGAG35650.046.430418
CAACGCA38750.042.7574655
AACGCAG40000.041.382856
GCAGAGT42000.039.4105769
GTACATG34450.031.204241
TACATGG35000.030.6671942
GTATACG950.004841947530.3775651
GAATACG3350.030.0758179
ACCGTCG1050.00799155627.4160548
GTGCTAG6100.026.0201281
GGTATCA28000.025.7666841
CATGGGG20300.025.5500834
CAGAGTA43050.024.9084879
AGGGGTG12450.024.880265
TATAGTG29050.023.7825987
Produced by FastQC (version 0.11.5)