FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699549
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36750.21623383615300296No Hit
ATATTATGCAGCGTCCGTGCAGGGGCGGTTCTCCATGTCCAGAGACAATT26840.1579242493155537No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24530.14433240818593637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.10991151181872368No Hit
GCATAATATGTATTGGTGCCACTGTCACTAATACCTGAGACCCACTCCAG18510.10891124645420638No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18200.10708723314243956No Hit
ATTTATTACTGCGCACGGACCCACTGGAACGTCGGAGAGGGTTGGTTCGG17450.10267429771074561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17350.10208590631985309No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37050.080.302891
ATCAACG45000.064.4828953
TCAACGC45550.063.704294
TATCAAC47450.061.7604142
CAACGCA48800.059.461695
AACGCAG51250.056.6174666
CGCAGAG59700.048.602348
GCAGAGT68700.042.4448249
ACGCAGA69050.042.125417
GGTATCA30400.036.019631
GTACATG49800.030.3780041
TACATGG50000.029.3773212
ACATGGG49450.029.1216343
GTATACG1050.00795354427.4435331
CAGAGTA66850.027.1409829
CATGGGG29000.023.5872654
CAGCGCT7050.023.4890379
CATGGGA26500.023.3670884
GTACACG10050.022.9264055
TACACGG10950.021.6989676
Produced by FastQC (version 0.11.5)